摘要
探讨洋川芎内酯Ⅰ在血管内皮细胞模型中的促血管生成作用,并初步探索其作用机制。用MTT方法考察洋川芎内酯I对人微血管内皮细胞(HMEC-1)细胞活力的影响;用管腔形成实验观察洋川芎内酯Ⅰ对人微血管内皮细胞管腔结构形成的影响;用血管生成抗体芯片技术检测洋川芎内酯Ⅰ处理后人微血管内皮细胞中多种血管生长因子的变化情况,探寻其可能的作用机制。结果表明,洋川芎内酯Ⅰ在浓度等于或低于50μmol/L时对HMEC-1细胞活力无明显影响。管腔形成实验发现洋川芎内酯Ⅰ组管腔长度和支点数目较空白对照组均明显增多,说明洋川芎内酯I能显著促进人微血管内皮细胞形成管腔结构,且表现出一定的浓度依赖性趋势。作用机制研究表明,洋川芎内酯I对胎盘生长因子(PIGF)有明显的上调作用。这些结果提示,洋川芎内酯Ⅰ可能是通过上调PIGF而促进人微血管内皮细胞的血管生成。
The present study investigated the proangiogenesis effect of senkyunolide Ⅰ(CX-1) in human microvascular endothelial cells(HMEC-1) model and the possible mechanism involved.The effect of CX-1 on HMEC-1 cell viability was tested by MTT.The proangiogenesis effect of CX-1 was mea-sured by tube formation assay in HMEC-1 cells.Meanwhile,the effect of CX-1 on angiogenic cytokines by angiogenesis antibody arrays and Western blotting was also investigated.The results showed that CX-1 had no significant effect on HMEC-1 cells viability when concentrations lower than or equal to 50 μmol/L and CX-1 can remarkably increase microvessel tube length and tube branching points in a concentration-dependent way compared to the control.Meanwhile,the results showed that CX-1 could up-regulate PIGF expression in HMEC-1 cells.It can be concluded that Senkyunolide Ⅰ promotes endothelial cells angiogenesis through PIGF signaling pathway.
出处
《药物生物技术》
CAS
CSCD
2011年第3期211-214,共4页
Pharmaceutical Biotechnology