百草枯中毒大鼠肾脏病理变化及caspase-3的表达

Pathologic changes and expression of caspase-3 in kidneys of paraquat poisoned rats

目的观察急性百草枯(PQ)中毒大鼠肾脏病理变化和肾脏caspase-3的表达,探过PQ中毒肾损伤病理生理机制。方法 36只健康SD大鼠随机分为对照组和染毒组,染毒组合予80 mg/kg PQ一次性灌胃染毒建立急性中毒模型,对照组等量生理盐水灌胃。分别观察24、48、72 h肾组织病理学变化,同时采用免疫组织化学法观察caspase-3蛋白表达。计量资料以均数±标准差(x±s)表示,组间单因素方差分析,结果以P<0.05为差异有统计学意义。结果对照组肾组织结构清晰,染毒组肾组织结构清晰度明显下降,染毒24 h可见充血、水肿等病理变化,随时间延长而加重;肾小球亦可受累。对照组caspase-3蛋白多不表达;染毒组染毒24 h在皮质部肾小管上皮细胞的胞膜及胞浆中caspase-3蛋白呈阳性表达,免疫组织化学评分(IHS)升高,与对照组各时间点比较,差异均有统计学意义(P<0.05)。结论 caspase-3蛋白高表达提示其可能参与PQ中毒肾损伤病理生理过程。

Objective To observe the pathological change and the expression of caspase-3 in the kidneys of paraquat poisoned rats for exploring the mechanism of renal damage caused by paraquat.Methods Thirty-six Sprague-Dawley（SD）rats were randomly divided into two groups,i.e.the control group and the paraquat group.Rats in the paraquat group were administered intragastrically with 80 mg/kg paraquat,and rats in the control group were only treated with normal saline.Then,examined the histopathological changes in kidney,meanwhile,the caspase-3 expression in kidney was also detected by immunohistochemistry（IHC）at 24,48 and 72 h after administration,respectively.Results In paraquat poisoned rats,obvious renal tubule lesions,including cellular swelling,tubular stenosis,mesenchymal congestion were observed.Pathologic changes gradually aggravated until the end of the study;glomerules might also be involeved.Compared with the control group,expression of caspase-3 in the kidneys of paraquat poisoned rats was obviously increased at all the time points（P〈0.05）.Conclusions The results suggested that caspase-3 might be involved in the pathogenesis of renal damage caused by paraquat.