应用RP-HPLC法测定不同产地中桔梗皂甙D

Determination of Platycodin Dof Radix Platycodiin Different Places by RP- HPLC

用反相高效液相色谱（ＲＰ－ ＨＰＬＣ） 分析方法对常用中药桔梗中主要有效成分桔梗皂甙Ｄ（ｐｌａｔｙｃｏｄｉｎＤ） 进行了研究，考察了流动相对分离的影响，选择以Ｃ１８ 为色谱分析柱，乙腈－ 磷酸盐缓冲液为流动相，检测波长为２１０ ｎｍ ，进样量在２－０ ～３２－０ μｇ 之间呈良好线性关系，平均回收率为９８－１３ ％ ，ＲＳＤ 为２－３ ％ 。

This paper deals with a quantitative study on platycodin Dby RP- HPLC-The analyt icalcolumn used is Microsorb C18-The mobile phase was CH3CN- KH2PO4(0-05mol/L)(30  70) ,pH 3-1 ,regulated by H3PO4-Flow rate was 0-7 m L/min-The detection wavelength was 210 nm-The linear ranges of platycodin D was 2-0 ～32-0 μg-The accuracy of method was checked by recoveries-The recovery of platycodin D was 98-13 % -The analytical results showed that the content of platycodin Din samples showed great different between different areas-