摘要
用反相高效液相色谱(RP- HPLC) 分析方法对常用中药桔梗中主要有效成分桔梗皂甙D(platycodinD) 进行了研究,考察了流动相对分离的影响,选择以C18 为色谱分析柱,乙腈- 磷酸盐缓冲液为流动相,检测波长为210 nm ,进样量在2-0 ~32-0 μg 之间呈良好线性关系,平均回收率为98-13 % ,RSD 为2-3 % 。
This paper deals with a quantitative study on platycodin Dby RP- HPLC-The analyt icalcolumn used is Microsorb C18-The mobile phase was CH3CN- KH2PO4(0-05mol/L)(30 70) ,pH 3-1 ,regulated by H3PO4-Flow rate was 0-7 m L/min-The detection wavelength was 210 nm-The linear ranges of platycodin D was 2-0 ~32-0 μg-The accuracy of method was checked by recoveries-The recovery of platycodin D was 98-13 % -The analytical results showed that the content of platycodin Din samples showed great different between different areas-
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
1999年第4期35-38,共4页
Journal of Jilin Agricultural University
基金
国家"九五"攻关计划资助!(96 - 903 - 02 - 01)