茶皂素毒性刺激性试验及抑菌作用研究

Toxicity,irritation and bacteriostasis of tea saponin

为探讨茶皂素的毒性、刺激性及抑菌作用,将不同质量浓度的茶皂素单次涂抹于家兔背部完整/破损脱毛皮肤上,观察皮肤红斑及水肿情况;取不同质量浓度的茶皂素滴于家兔眼内,观察其刺激性;取适量不同质量浓度的茶皂素涂抹于大鼠背部完整皮肤上,观察毒性反应;采用滤纸片法,测定茶皂素对常见致病菌的抑菌作用。结果表明:质量浓度为40 mg/mL的茶皂素对皮肤有轻微的刺激1,6 mg/mL的茶皂素对眼有轻微刺激;茶皂素无明显毒性作用;茶皂素对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、酵母菌有较明显的抑制作用,对白色念珠菌有一定的抑制作用,对绿脓杆菌无抑制作用。

In order to investigate the irritation, acute toxicity and bacteriostasis of tea saponin, different concentrations of tea saponin were smeared on the intact and damaged skin of rabbits back to observe the skin erythema and edema, different concentrations of tea saponin were droped in rabbits eyes to observe the irritation, different concentrations of tea saponin were smeared on the intact skin of rats back to observe the toxicity, and filter paper method was used to observe the bacteriostasis of tea saponin in common pathogens. The results showed that 40 mg/mL of the tea saponin had slight irritation to skin, 16 mg/mL of the tea saponin had slight irritation to the eyes; the tea saponin had no obvious toxicity; the tea saponin had strong inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and yeast, and had certain inhibitory effect on Canclida albicans and had no inhibitory effect on Pseudomonas aeruginosa （pa）.