HPLC测定芹菜籽提取物中木犀草素含量

Determination of Luteolin in Celery Seed Extract by HPLC

目的建立高效液相色谱法测定芹菜籽提取物中木犀草素的含量。方法采用Apollo C18柱（4.6 mm×250 mm,5μm）;流动相为乙腈-0.1%磷酸溶液（30∶70）;流速为1.0 mL.min-1;进样量为20μL;检测波长为269 nm;柱温为30℃。结果木犀草素浓度线性范围为0.50~10.0μg.mL-1（r=0.999 8）,3个浓度的加样回收率分别为100.2%,100.1%和101.2%,RSD分别为1.02%,1.91%和1.75%。结论本法操作简单、准确、专属性强,可作为芹菜籽提取物质量控制的方法。

OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C18 column （4.6 mm×250 mm, 5 μm）. The mobile phase was consisted of acetonitrile-0.1% phosphoric acid （30 ∶7 0）, and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg.mL-1 for luteolin （r=0.999 8）. The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.