HPLC同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂酰胆碱含量

Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC

目的建立同时测定大豆磷脂中磷脂酰胆碱（PC）和溶血磷脂酰胆碱（LPC）含量的方法。方法采用高效液相色谱法,色谱柱为Phenomenex silica柱（4.6 mm×150 mm,5μm）,流动相为正己烷-异丙醇-水（5∶80∶20）,流速：0.7 mL.min-1,检测波长：206 nm,柱温：25℃。结果 PC在0.4~2.4 mg.mL-1内呈良好的线性关系,平均回收率为99.4%,RSD为0.40%（n=6）;LPC在10~60μg.mL-1内呈良好的线性关系,平均回收率为98.0%,RSD为1.65%（n=6）。结论本试验方法简便、重复性好、专属性强,可作为大豆磷脂中PC,LPC的检测方法。

OBJECTIVE To establish an HPLC method for simultaneously determining phosphatidylcholine （PC）, lysophosphatidylcholine （LPC） in soybean phospholipids. METHODS The HPLC system consisted of a Phenomenex silica column（4.6 mm×150 mm, 5 μm）, a mobile phase of n-hexane-isopropanol-water（5∶80∶20）. The flow rate was 0.7 mL.min-1. Column temperature was set at 25 ℃ and the wavelength of detector was set at 206 nm. RESULTS The calibration curve was linear in the range of 0.4-2.4 mg.mL-1 for PC and 10-60 μg.mL-1 for LPC, respectively. The average recovery for PC was 99.4% and RSD was 0.40%（n=6）. The average recovery for LPC was 98.0% and RSD was 1.65%（n=6）. CONCLUSION The method is simple, rapid and specially. It can be used for the determination of PC and LPC in soybean phospholipids.