三维荧光光谱法测定水中氯苯的研究

Study of Three-Dimensional Fluorescence Spectra for Measuring Chlorobenzene in Water

对三维荧光光谱法测定水中氯苯（简称CB）进行了研究。研究表明,CB的三维荧光谱图只有一个荧光峰,该峰位于激发波长（eλx）210~240 nm、发射波长（eλm）330~370 nm范围内.当CB溶液浓度为0.002~0.05 mg.L-1时,eλx/λem为225/340 nm处荧光强度最大。在此波长处,荧光强度与浓度呈很好的线性相关,相关系数为0.999 67,表明三维荧光光谱法可用于定量分析水中的CB。该方法在置信水平为90%时的检出限为3.68×10-6mg.L-1,标准偏差为0.04%。

Quantitative analysis of chlorobenzene（CB） in water by three-dimensional fluorescence spectrometry was discussed in the present paper.The study showed that there is only one fluorescence peak for CB which lies in the range of excitation wavelength（λex） 210~240 nm and emission wavelength（λem） 330~370 nm.When measuring CB solution of concentration 0.002~0.05 mg·L-1,the fluorescence intensity was the strongest as λex/λem was 225/340 nm,which presented linear correlation with concentration,and the related coefficient was 0.999 67.The study proved that three-dimensional fluorescence spectrometry can be adopted for quantitative analysis of CB in water.With this method,the detection limit was 3.68×10-6 mg·L-1 and the standard deviation was 0.04% at 90% confidence level.