血必净注射液对热射病大鼠保护机制的研究

The protective role of Xuebijing injection to heat stroke in rats

目的检测热射病(HS)大鼠凝血功能、生化指标及细胞因子的变化,探讨血必净注射液(XBJ)在对抗HS中的作用机制。方法清洁级成年雄性SD大鼠24只,随机分为3组(n=8):正常对照(NC)组、生理盐水处理(NS-HS)组、血必净治疗(XBJ-HS)组。3组分别于热应激开始的0、47和57min经股动脉抽血2.3ml,检测大鼠血浆凝血指标(APTT、PT、FDP、D-二聚体)、生化指标(Cr、BUN、AST、ALT、ALP)以及细胞因子(IL-1β、IL-6、TNF-α)浓度,并于热应激57min后处死大鼠,取肝脏制作石蜡切片,HE染色光镜观察。结果两组(NS-HS组和XBJ-HS组)热应激动物均出现系统炎症反应且凝血活性增强,表现为细胞因子和凝血指标的显著增高,同时存在组织缺血和损伤,主要表现为生化指标的显著升高。血必净注射液可以显著降低热射病大鼠血浆的凝血指标、生化指标以及细胞因子浓度(P<0.05),减轻肝脏的病理损害。结论早期给予血必净注射液干预治疗,可以通过降低系统炎症反应、凝血活性以及减轻组织缺血和损伤的程度,延长热射病大鼠的生存时间。

Objective To analyze the variations in coagulation function,biochemical indicator and cytokines of the rats suffering from heat stroke,and explore the protective effects of Xuebijing injection against heat stroke.Methods Twenty-four SPF male SD rats were randomly divided into the following three groups（8 each）： normal control（NC group）,saline treatment for heat stroke（NS-HS group）,and Xuebijing injection treatment for heat stroke（XBJ-HS group）.2.3ml blood samples were drawn from femoral artery at 0,47 and 57 min after the initiation of heat exposure to measure the plasma levels of APTT,PT,FDP,D-dimers,Cr,BUN,AST,ALT,ALP,IL-1β,IL-6 and TNF-α.The rats were sacrificed immediately at 57 min after the initiation of heat exposure,and the liver was then harvested and fixed with formalin.The paraffin section of liver was stained by HE and observed under the light microscope.Results Rats in both NS-HS and XBJ-HS group displayed an excessive systemic inflammation and excessive coagubility,as well as the tissue ischemia and injury.Excessive systemic inflammation during heat stroke was shown by increased levels of IL-1β,IL-6,and TNF-α in plasma.Hypercoagubility were shown by increased plasma levels of APTT,PT,FDP,and D-dimers.Tissue ischemia and injury was shown by increased levels of creatinine,BUN,AST,ALT,and ALP in plasma.Xuebijing injection significantly decreased the plasma levels of APTT,PT,FDP,D-dimers,Cr,BUN,AST,ALT,ALP,IL-1β,IL-6,and TNF-α（P0.05）,and alleviate the pathological lesion of liver.Conclusions Early administration of Xuebijing injection before the onset of heat-stress can prolong the survival time of rats with heat stroke by alleviating systemic inflammation,attenuating coagulability and tissue ischemia/injury.