上皮性卵巢癌中尿激酶型纤溶酶原激活因子的表达及其与肿瘤血管生成的关系

Relationship between expression of urokinase-type plasminogen activator and tumor angiogenesis in epithelial ovarian carcinoma

目的探讨尿激酶型纤溶酶原激活因子(uPA)在上皮性卵巢癌组织中的表达及其与肿瘤血管生成的关系。方法采用免疫组化SP法检测uPA在上皮性卵巢癌(n=85)、上皮性卵巢交界性肿瘤(n=16)、上皮性卵巢良性肿瘤(n=39)及正常卵巢组织(n=24)中的表达情况,以CD34标记上皮性卵巢癌中的微血管,采用人工计数测定其微血管密度(MVD),分析uPA表达与MVD的相关性,并对85例上皮性卵巢癌患者进行随访。结果 uPA在上皮性卵巢癌中的阳性表达率最高。uPA及MVD的表达与上皮性卵巢癌的分化程度、临床分期、淋巴转移、大网膜转移及5年生存率有关(P<0.05),且uPA在上皮性卵巢癌中的表达与MVD呈显著正相关(r=0.56,P=0.02)。结论 uPA可促进上皮性卵巢癌的血管生成,并参与肿瘤的发生发展及侵袭转移。uPA表达及MVD可作为判断上皮性卵巢癌侵袭、转移及预后的重要指标。

Objective To explore the expression of urokinase-type plasminogen activator（uPA） in epithelial ovarian carcinoma（EOC） and the relationship of the expression to microvessel density.Methods SP immunohistochemical staining was performed to determine the expression of uPA in ovarian cancer（85 cases）,borderline ovarian tumor（16 cases）,benign ovarian tumor（39 cases） and normal ovarian tissue（24 cases）.Microvessels in ovarian cancer were marked by CD34,and microvessel density（MVD） was determined by direct count.The relationship between uPA and MVD was analyzed.The 85 patients with epithelial ovarian cancer were followed up.Results The highest positive rate of uPA existed in the patients with EOC.There was a correlation between the expression of uPA and MVD and the differentiation of EOC,clinical stage,lymphatic metastasis,omental metastasis,and 5 years survival rate.A significant positive correlation was found between the expression of uPA in EOC and MVD（r=0.56,P=0.02）.Conclusion uPA may promote the angiogenesis of EOC,and participate in the occurrence,development,invasion and metastasis of ovarian cancer.The detection of uPA and MVD may be used as an indicator of biological behaviors and prognosis of ovarian cancer.