期刊文献+

三七总皂苷对前列腺癌PC-3细胞增殖及迁移的抑制作用 被引量:10

Inhibition of the Proliferation and Migration of Human Prostate Cancer PC-3 Cells by Total Panax Notoginseng Saponins
下载PDF
导出
摘要 目的:探讨三七总皂苷对人前列腺癌PC-3细胞增殖及迁移的抑制作用,初步研究该药物的作用机制,并为前列腺癌的药物治疗及扩展tPNS的临床应用提供实验依据。方法:采用MTT实验、细胞计数、伤口愈合实验等方法检测tPNS对PC-3细胞增殖及迁移的影响;采用Western blot及明胶酶图分析等方法对增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、血管细胞间黏附分子1(vascular cell adhesion molecule 1,VCAM-1)及迁移相关蛋白基质金属蛋白酶2(matrix metalloproteinases 2,MMP-2)等进行检测。通过检测p38 MAPK及ERK途径的磷酸化变化,探讨tPNS对PC-3细胞作用的可能信号途径。结果:tPNS可抑制PC-3细胞增殖,随着tPNS浓度(200、400、800mg/L)的增加,对PC-3细胞的抑制率分别增加至13.0%、29.5%和35.9%(P<0.05)。tPNs下调PC-3细胞增殖标志物PCNA的表达水平,该效应呈量效及时效关系。tPNs(200、400、800mg/L)可显著抑制PC-3细胞迁移。tPNS还可显著下调迁移相关蛋白MMP-2及黏附分子VCAM-1的表达水平。tPNS可显著增加p38 MAPK的磷酸化水平,但对ERK磷酸化影响不明显。结论:tPNS抑制人前列腺癌PC-3细胞增殖及迁移活性,这些生物学作用可能与该药抑制PCNA、VCAM-1、MMP-2的表达及激活p38 MAPK信号通路有关。 Objective: To investigate the effects of total Panax notoginseng saponins ( tPNS ) on the proliferation and migration of PC-3 cells, and to provide experimental evidence for the use of tPNS in treating prostatic cancer. Methods: MTT, cell counting, and wound-healing assays were performed to analyze the effects of tPNS on the proliferation and migration of PC-3 cells. The expression of proliferating cell nuclear antigen ( PCNA ), vascular cell adhesion molecule 1 ( VCAM- 1 ), and matrix metalloproteinases 2 ( MMP-2 ) was determined using Western blot analysis or zymography. Activation of p38 MAPK and ERK was also detected by Western blot analysis after tPNS treatment. Results: tPNS inhibited the proliferation of PC-3 ceils. The inhibition ratios were 13.0%, 29.5%, and 35.9% after the PC-3 cells were treated with tPNS ( 200, 400, and 800 mg/L, respectively ) ( P〈 0.05 ). PCNA expression was also inhibited in dose- and time-dependent manners. These results indicated that tPNS could significantly inhibit PC-3 cell proliferation via downregula- tion of PCNA. The migration of PC-3 cells was inhibited after treatment with tPNS. The Western blot and zymography results suggested that tPNS significantly suppressed the expression of the migration-related protein MMP-2. VCAM-1 was also downregulated after tPNS treatment in PC-3 cells. The aforementioned results indicated that tPNS inhibited PC-3 cell migration by suppressing MMP-2 and VCAM-1 expression. The phosphorylation level of the p38 MAPK pathway was dramatically increased at 0.5, 1, 3, and 6 h after tPNS treatment ( 400 mg/L ). However, phosphorylation of the ERK pathway was unaffected. Conclusion: tPNS can inhibit the proliferation and migration of PC-3 cells. The underlying mechanism may be related to the expression of PCNA, VCAM-1, and MMP-2 and activa- tion of the p38 MAPK pathway.
出处 《中国肿瘤临床》 CAS CSCD 北大核心 2011年第11期638-641,共4页 Chinese Journal of Clinical Oncology
基金 河北省普通高等学校强势特色学科项目资助~~
关键词 前列腺癌 三七总皂苷 增殖 迁移 Prostate cancer Total panax notoginseng saponins Proliferation Migration
  • 相关文献

参考文献10

  • 1尚西亮,傅华群,刘佳,李学东.三七总皂甙对人肝癌细胞的抑制作用[J].中国临床康复,2006,10(23):121-123. 被引量:35
  • 2Chen W,Dang Y,Zhu C.Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study[J].Chin Med,2010,5:12.
  • 3Yang ZG,Sun HX,Ye YP.Ginsenoside Rd from Panax notoginseng is cytotoxic towards HeLa cancer cells and induces apoptosis[J].Chem Biodivers,2006,3(2):187-197.
  • 4Wang CZ,Luo X,Zhang B,et al.Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells[J].Cancer Chemother Pharmacol,2007,60(1):69-79.
  • 5郑红兵,张素丽.Cyclin G_1和PCNA在子宫肌瘤中的表达及临床意义[J].中国肿瘤临床,2007,34(1):34-37. 被引量:3
  • 6Wong CP,Bray TM,Ho E.Induction of proinflammatory response in prostate cancer epithelial cells by activated macrophages[J].Cancer Lett,2009,276(1):38-46.
  • 7Shariat SF,Karam JA,Walz J,et al.Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers[J].Clin Cancer Res,2008,14(12):3785-3791.
  • 8熊枝繁,李杰,曹仕琼,刘浩,赵志辉,李丽,李正英,吴燕茹,熊莉娜.CD147和MMP-2在大肠癌组织中的表达及血清水平变化的意义[J].中国肿瘤临床,2008,35(18):1064-1067. 被引量:3
  • 9赵秀兰,杜静,赵学铭,沈军,倪春生,赵楠,孙保存.VEGF和MMP-2表达与肝细胞肝癌复发和预后的关系[J].中国肿瘤临床,2008,35(16):934-937. 被引量:7
  • 10Kim EK,Choi EJ.Pathological roles of MAPK signaling pathways in human diseases[J].Biochim Biophys Acta,2010,1802(4):396-405.

二级参考文献32

  • 1肖春花,冯玉梅,李晓青,郝希山.基质金属蛋白酶-2和纤维粘连蛋白mRNA表达与乳腺癌转移相关性的探讨[J].中国肿瘤临床,2005,32(8):424-427. 被引量:3
  • 2郭荣平,钟崇,石明,张昌卿,韦玮,张亚奇,李锦清.血管内皮生长因子和基质金属蛋白酶-2在肝细胞肝癌中的表达及临床意义[J].中华肿瘤杂志,2006,28(4):285-288. 被引量:27
  • 3Wakabayashi C,Murakami K,Hasegawa H,et al.An intestinal bacterial metabolite of ginseng, protopanaxadiol saponins has the ability to induce apotosis in tumor cells. Biachem Biophys Res Commun 1998,246(3):725-30
  • 4Carruba G,Webber MM,Quader ST,et al.Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 2002, 50(2) :73-82
  • 5Trosko JE, Madhukar BV, Masten SJ.Endogenous and exogenous modulation of gap junctional intercellular communication:Toxicological and pharmacological implications. Life Sci 1993,53(1): 1-19
  • 6Huang RP,Fan Y,Hossain MZ,et al.Reversion of the neoplastic phenotype of human glioblastoma cells by connexin43 (cx43).Cancer Res 1998 , 58(22): 5089-96
  • 7Yamauchi H, Katayama K, Ueno M, et al. Involvement of p53 in 1-beta-D-arabinofuranosylcytosine-induced rat fetal brain lesions[J]. Neurotoxicol Teratol, 2004, 26(4):579-586
  • 8Yamauchi H, Katayama K, Ueno M, et al. Involvement of p53 in 1-beta-D- arabinofuranosylcytosine-induced trophoblastic cell apoptosis and impaired proliferation in rat placenta [J]. Biol Reprod, 2004, 70(6):1762- 1767
  • 9Katayama K, Ohtsuka R, Takai H, et al. Expression of p53 andits transcription target genes mRNAs in the ethylnitrosourea-induced apoptosis and cell cycle arrest in the fetal central nevous system[J]. Histol Histopathol, 2002, 17(3):715-720
  • 10Baek WK, Kim D, Jung N, et al. Increased expression of cyclin G1 in leiomyoma compared with normal myometrinm [J]. Am J Obstet Gynecol, 2003, 188(3):634-639

共引文献44

同被引文献266

引证文献10

二级引证文献72

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部