血链球菌细菌素对唾液生物膜中牙龈卟啉单胞菌和具核梭杆菌拮抗作用的实验研究

Study of Antagonistic Effects of Sanguicin on Salivary Biofilm of Porphyromonas Gingivalis and Fusobacterium Nucleatum in Vitro

目的:提取血链球菌标准菌株(ATCC10556)细菌素,研究其对唾液生物膜中牙龈卟啉单胞菌和具核梭杆菌的拮抗作用。方法:通过超声破碎,高速离心,盐析等方法提取血链球菌细菌素;采用二倍稀释法,测定血链球菌细菌素对浮游状态下牙龈卟啉单胞菌和具核梭杆菌的最小抑制浓度(MIC);在体外建立牙龈卟啉单胞菌和具核梭杆菌唾液生物膜模型,利用激光共聚焦扫描电镜观察该生物膜中细菌活性的变化。结果:血链球菌细菌素对浮游状态下的牙龈卟啉单胞菌和具核梭杆菌的MIC为0.5 g/L;使用血链球菌细菌素作用牙龈卟啉单胞菌和具核梭杆菌生物膜,活菌百分比显著下降,与对照组相比具有显著性差异(P<0.05)。结论:血链球菌细菌素对浮游状态下和生物膜中的牙龈卟啉单胞菌和具核梭杆菌生长均有显著的抑制作用。

Objective： To investigate the effects of sanguicin extracted from streptococcus sanguis on salivary biofilm of Porphyromonas gingivalis （P. g） and Fusobacterium nucleatum （F. n） in vitro. Methods： Ultrasonic, centrifugation and ammonium sulfate were used to extract sanguicin. The minimal inhibitory concentration（MIC） of sanguicin against P. g and F. n was measured by the broth dilution technique. Biofilms formed by P. g and F. n were observed by confocal laser scan microscope（CLSM） to determine the percentage of vital cells. Results.. The MIC of Sanguicin on planktonic P. g and F. n was 0.5mg/ml and the percentage of vital bacteria of biofilm reduced significantly after using Sanguicin （P〈0.05）. Conclusion： Sanguicin is an effective anti-P, g and F. n agent in both planktonic and the biofilms .