正常人、牙龈炎和牙周炎患者龈沟液内一氧化氮含量的检测

Detection of the Level of Nitric Oxide in Gingival Crevicular Fluid in Healthy Individuals,Gingivitis and Chronic Periodontitis Patients

目的:检测正常人和牙周病患者龈沟液中NO含量,探讨NO在牙周病发病过程中的作用。方法:选择牙周健康组20例,牙龈炎组22例,慢性牙周炎组32例,分别采集龈沟液标本,免疫荧光法检测龈沟液内NO的含量。结果:慢性牙周炎患者和牙龈炎患者龈沟液内NO含量与牙周健康组相比均有高度显著性差异(P<0.01),慢性牙周炎患者龈沟液内NO含量与牙龈炎组相比有高度显著性差异(P<0.01)。结论:牙周健康者、牙龈炎患者、慢性牙周炎患者龈沟液中能检测出NO的存在,NO参与了慢性牙周炎的发展过程,龈沟液内NO含量与慢性牙周炎炎症程度密切相关。

Objective： To observe the level of nitric oxide in gingival crevieular fluid （GCF） in normal group and periodontitis group, and to study the possible role of NO in the periodontal disease. Methods： The GCF samples were collected in 20 periodontally healthy individuals, 22 gingivitis and 32 chronic periodontitis patients. The level of nitric oxide in gingival crevicular fluid was measured by immunofluorescence. Results： The nitric oxide in GCF was a significant difference in chronic periodontitis group and gingivitis group than that in control group（P〈0.01）. The nitric oxide in GCF was significantly different in chronic periodontitis group compared to that in gingivitis group （P〈0.01）. Conclusion： Nitric oxide exists in GCF of patients with chronic periodontitis and gingivitis and periodontally healthy individuals. Nitric oxide plays an important role in the development of chronic periodontal disease. The level of nitric oxide in GCF has close relation with degree of inflammation.