应用FTIR光谱技术推断死亡时间

To estimate the postmortem interval using FTIR spectroscopy

目的应用傅立叶变换红外(FTIR)光谱技术分析大鼠死后组织、人体离体组织随死亡时间推移的化学降解过程,为死亡时间推断的研究提供新的途径与方法。方法大鼠断颈处死后置于4℃、20℃、30℃环境,在不同死亡时间点提取大鼠不同组织;收集有同样死亡时间的人尸体组织,离体取材,并运用FTIR光谱仪测定不同化学基团随死后和离体后的变化。结果随着死亡时间的推移,大鼠组织、人离体组织的FTIR光谱主要吸收峰峰位没有明显变化,而其峰强随着死亡时间增加呈现出4种不同的变化方式,增加、下降、稳定、波动,且不同峰强比显示了相似的时间变化趋势。结论 FTIR光谱技术为死亡时间推断提供了一种崭新的技术。

Objective The rat and human tissues were analyzed using FTIR spectroscopy technique after death and the study can provide a new method to estimate postmortem interval. Methods The rats were sacrificed by cervical dislocation and the cadavers were kept at 4,20,30℃ in a controlled environment chamber. The tissues were sub-sampled from different organs at different time points postmortem. The human tissues were taken at intervals after death. The spectra were collected to explore the changes of different chemical functions. Results The absorbance bands position of rat and human showed no obvious changes. The absorbance band intensity showed four change ways：increase, decrease, stable and instability. The different absorbance band ratios showed similar changes tendency. Conclusion FTIR can provide a new method to estimate the postmortem interval.