乙型肝炎外周血Th1/Th2及Tc1/Tc2细胞失衡的临床意义研究

Clinical Significances of Th1/Th2 and Tc1/Tc2 Cells Imbalance in Peripheral Blood of Patients with Hepatitis B

目的探讨外周血Th1/Th2、Tc1/Tc2细胞失衡在乙型肝炎中的临床意义。方法 采用流式细胞术分析乙型肝炎患者外周血不同T淋巴细胞亚群中细胞因子γ-干扰素(IFN-γ)和白介素4(IL-4)表达率的变化,分别比较急慢性乙型肝炎以及慢性轻、中、重度肝炎Th1/Th2、Tc1/Tc2的变化。结果慢性乙型肝炎Th2、Tc2细胞均高于急性乙型肝炎(t=-3.54,P=0.001;t=-2.27,P=0.026);相对于轻度组,中度以及重度组Th1、Tc1均有升高(P<0.01);相对于中度组,重度组Th1、Tc1亦有增高(P<0.05)。结论乙型肝炎外周血Th2、Tc2细胞与炎症慢性化有关;随着慢性乙型肝炎活动程度的加剧,Th1、Tc1细胞逐渐增多。

Objective To explore the clinical significances of Th1/Th2 and Tc1/Tc2 cells imbalance in patients with hepatitis B. Methods The different T cell subtypes（IFN-γ and IL-4） were assessed by flow cytometry.The Th1/Th2 and Tc1/Tc2 levels between acute and chronic hepatitis B and among slight,moderate,and severe chronic hepatitis B were compared. Results The Th2 and Tc2 levels were significantly higher in chronic hepatitis B than in acute chronic hepatitis B（t=-3.54,P=0.001;t=-2.27,P=0.026）.Compared with the slight group,Th1 and Tc1 levels were significantly higher in the moderate and severe groups（P〈0.01）.Compared with the moderate group,Th1 and Tc1 levels were significantly higher in the severe group（P〈0.05）. Conclusion Th2 and Tc2 levels are associated with chronic inflammation in hepatitis B cases.Th1 and Tc1 levels increase with the increased inflammatory activity in hepatitis B cases.