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1994-2006年深圳市分离的B型流感病毒分子进化研究 被引量:2

The study on molecular evolution of influenza virus B isolated in Shenzhen from 1994 to 2006
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摘要 目的通过对深圳市分离的B型流感病毒HAl分子系统进化分析,初步了解深圳市B型流感病毒的流行变异规律。方法选取深圳市1994--2006共13年间分离的50株B型流感毒株,通过RT-PCR将其HAl基因片段扩增后进行序列解析,然后,通过MEGA等软件对序列进行分子进化分析。结果1994--2006年间深圳市流行的B型流感病毒分为Yamagata和Victoria两个亚系,两系的毒株分别是不同年份的主要流行株。两个亚系的HAl分子具有1个糖基化位点的差异,在4个抗原决定簇区域也分布有多个氨基酸位点的变异。结论1994--2006年间深圳市B型流感病毒的两个亚系分别在不同年份主导流行,但变异均比较缓慢。 Objective To study the prevalence and variation of influenza B viruses of Shenzhen. Methods Fifty strains influenza B viruses in Shenzhen from 1994 to 2006 were selected. HA1 gene were amplified by RT-PCR and sequenced. Phylogenetic analysis of HA1 was conducted by MEGA program. Resuits The influenza B viruses of Shenzhen were divided into Yamagata and Victoria lineage. The two lineages prevailed respectively in different years from 1994 to 2006. The variance of glycosylation site and some mutations of antigenic determinants were detected in the two lineages. Conclusion The viruses of Yamagata and Victoria lineage prevailed respectively in different years in Shenzhen but the mutation rates of the two lineages were slowly.
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2011年第5期398-402,共5页 Chinese Journal of Microbiology and Immunology
关键词 B型流感病毒 系统进化 分子变异 Influenza virus B Phyletic evolution Molecular mutation
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