内源性CNT在动物血液及组织中的变化与分布

DISTRIBUTION OF ENDOGENOUS CNT IN ANIMAL

以豚鼠为实验动物，应用放射免疫测定方法研究了内源性ｃＡＭＰ 和ｃＧＭＰ 在动物血液中含量变化及组织分布。结果表明，正常情况下动物血液中ｃＡＭＰ 和ｃＧＭＰ的变化幅度不大， 二者含量基本稳定在２０７３８ ±０９６７ｐ ｍｏｌ／ ｍｌ 和５８１５ ±００３２ｐ ｍｏｌ／ ｍｌ；肝脏细胞中含ｃＡＭＰ 最多（８３２００ ±２１４６ｐｍ ｏｌ／ｇ） ，脂肪细胞中最少（５７６７ ±３６８ｐｍ ｏｌ／ｇ） ；ｃＧＭＰ 则以小肠分布最多（３６９７ ±１７０ｐ ｍｏｌ／ｇ） ，脂肪中最少（１９３ ±００５ｐｍ ｏｌ／ｇ） ，各组织或器官ｃＡＭＰ／ｃＧＭＰ 值在１８６５ ～７５０６ 范围内变化。

Using radioimmunoassay and other techniques, the distribution of endogenous cAMP, cGMP in Quinea pigs was studied. The results indicated that under normal condition the contents of cAMP and cGMP in the animal blood were not significantly changed (20 738±0 967pmol/ml and 5 815±0 032pmol/ml respectively). The content of cAMP in the liver was the highest(832 00±21 46pmol/g), and the lowest content was found in the adipose tissue (57 67±3 68pmol/g). The content of cGMP in small intestine was the highest (36 97±1 70pmol/g), and the lowest content was found in the adipose tissue(1 93±0 05pmol/g). The ratio of cAMP and cGMP in all of the tissues and organs changed in the range of 18 65～76 06.