粪产碱菌耐铵工程菌与水稻联合共生固氮作用

ASSOCIATION NITROGEN FIXATION OF RICE INOCULATED WITH AMMONIA RESISTANT ENGINEERING STRAIN( Alcaligenes faecalis )

粪产碱菌（ Ａａｅｃａｌｉｓｆａｅｃａｌｉｓ） 在ＬＷ 培养基下能产生植物激素ＩＡＡ。盆栽结果表明，接种Ａ．ｆａｅｃａｌｉｓ 显著提高了水稻的生长，接种Ａ１５０１ 和Ａ１５１３ 的水稻稻谷产量分别比未接种提高了８５％ 和１０３ ％ ，用１５ Ｎ 同位素稀释法估测水稻地上部总氮中来源联合固氮的百分率达９００ ％ 和１１５ ％ ，其结果与水稻地上部（ 茎叶及稻谷） 总氮量的增加幅度（８６ ％ 和１１６ ％ ）基本一致。研究还表明Ａ１５１３ 耐铵工程菌的产ＩＡＡ作用以及对水稻的生长和产量、联合固氮作用均优于原始菌株Ａ１５０１ 。

It showed that Alcaligenes faecalis could produce plant hormone (IAA) in LW medium. The pot experiment results showed that inoculation with A1501 and A1513 could promote the growth and grain yield of rice. Comparison with the non\|inoculation, the grain yeild of rice treated with A1501 and A1513 increased by 8 5% and 10 3% respectively. And %Ndfa of rice shoot and grain estimated by 15 N\|isotope dilution method was 9 00% and 11 5%, respectively, which was consistent with the increment of the total N(8 5% and 11 6%,respectively). The study indicated that ammonia resistant engineering strain A1513 had more stimulative effect on the growth of rice and grain yield than A1501.