HMGB-1在DR患者房水及血浆中水平变化分析

Investigation of HMGB-1 concentration in plasma and aqueous humor of patients with DR

目的:观察糖尿病性视网膜病变(diabetic retinopathy,DR)患者房水和血浆中高迁移率族蛋白-1(high-mobility groupbox-1,HMGB-1)的变化及其临床意义。方法:以西安交通大学第一附属医院眼科2010-08/12住院糖尿病(diabets mellitus,DM)患者为研究对象,按有无DR分为糖尿病无视网膜病变(non-diabetic retinopathy,NDR)组及DR组。DR组按病程分为单纯性糖尿病性视网膜病变(background diabetic retinopathy,BDR)组及增殖性糖尿病性视网膜病变(proliferative diabetic retinopathy,PDR)组。设正常对照组,对研究对象分别收集房水和血浆标本,共收集房水28例,血浆40例,均采用双抗体夹心ABC-ELISA法进行人HMGB-1定量ELISA测定。结果:DM患者房水中的HMGB-1浓度明显高于对照组(P<0.05),DM患者按DR病程分组时,HMGB-1浓度未见明显差异(P>0.05)。DM患者血浆中HMGB-1浓度与对照组比较未见明显差异(P>0.05)。结论:HMGB-1在DR的发生中可能起到重要作用,但与DR的病理进程无明显关系。

AIM：To observe the changes of high mobility group box-1 （HMGB-1） in plasma and aqueous humor of patients with diabetic retinopathy （DR） and its clinical significance.METHODS： Research objects were the patients with diabetic mellitus （DM） in the First Hospital of Xi’an Jiaotong University from August of 2010 to December of 2010. According to DR, the patients were divided into non-diabetic retinopathy （NDR） group and DR group. DR group was divided into background diabetic retinopathy （BDR） group, and proliferative diabetic retinopathy （PDR） group according to disease course. Normal control group was set, plasma group with 40 cases and aqueous humor group with 28 cases were collected, and they were analyzed by ABC-ELISA. RESULTS： The levels of aqueous humor HMGB-1 in patients with DM were significantly higher than those in control group （P〈0.05）. Of patients with diabetes, according to DR course, HMGB-1 concentration had not the obvious difference （P〉0.05）. HMGB-1 concentration in plasma of patients with diabetes had no obvious difference compared with control group（P〉0.05）.CONCLUSION： HMGB-1 may play an important role in the occurrence of DR, but it has no obvious relation with DR pathological process