TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究

TRB3 Mediates Homocysteine-induced Inhibition of Endothelial Cells Proliferation

目的研究TRB3对介导同型半胱氨酸的血管内皮细胞抑制的影响.方法用[3H]-胸腺嘧啶脱氧核苷掺入法测定细胞DNA合成率,基因表达测定采用RT-PCR,蛋白质磷酸化采用Western blotting.结果同型半胱氨酸处理人脐静脉内皮细胞导致细胞增殖被抑制;同型半胱氨酸诱导的内质网应激,导致CHOP和ATF4表达升高;同型半胱氨酸上调TRB3表达,导致Akt的去磷酸化.结论 TRB3是同型半胱氨酸介导的血管内皮细胞的细胞周期停滞的关键分子.

Objective To investigate the effect of TRB3 on homocysteine-induced inhibition of endothelial cells proliferation.Methods DNA synthesis in cells was determined by using -thymidine incorporation.The expression of tested genes was detected by real-time PCR.The phosphorylation of proteins was measured by Westren blotting.Results（1）Homocysteine inhibited the proliferation of human umbilical vein endothelial cells（HUVECs）.（2）Homocysteine induced endoplasmic reticulum stress,and up-regulated the expression of CHOP and ATF4.（3）Homocysteine up-regulated the expression of TRB3,resulting in dephosphorylation of Akt.Conclusion TRB3 is a critical molecule in homocysteine-mediated inhibition of proliferation in HUVECs.