尿液刺激在尿道术后瘢痕形成中的作用

Role of Continued Urine in the Formattion of Scar Tissue after Urethral Surgery

目的研究尿液刺激对尿道术后瘢痕形成的影响以及转化生长因子-β1(TGF-β1)在其中的作用.方法建立犬的尿道狭窄模型,分为对照组;无尿液刺激组;尿液刺激组.采集标本后用RT-PCR和western blot检测TGF-β1的表达情况,VanGieson染色检测瘢痕中胶原纤维变化情况.结果所有尿道损伤组TGF-β1 mRNA和蛋白的表达均较对照组升高(P<0.05);损伤组中尿液刺激组TGF-β1 mRNA和蛋白的表达较无尿液刺激组升高(P<0.05);尿道损伤组胶原纤维含量均较对照组升高(P<0.05);而尿液刺激组胶原纤维含量较无尿液刺激组升高(P<0.05).结论尿液刺激是尿道狭窄瘢痕形成的一个重要危险因素,它通过上调瘢痕组织TGF-β1水平得以促使狭窄加重.

Objective To study effect of continued urine on the formattion of scar tissue after urethral surgery,and the role of transforming growth factor β1（TGF-β1）in the process.Methods Experimental model of canine urethral stricture was established.Experimental grouping includes control group,injury group without continued urine stimulation and injury group with continued urine stimulation.The expression of TGF-β1 mRNA and protein was measured by RT-PCR and western blot respectively.Collagen fibers were observed by VanGieson staining.Results The expression of TGF-β1 and the content of collagen fibers were significantly increased in injury groups than in control group（P〈0.05）.Meanwhile,The expression of TGF-β1 and the content of levels fibers were significantly increased in injury groups with continued urine stimulation than in injury group without continued urine stimulation（P〈0.05）.Conclusion Continued urine stimulates the formation of scar tissue after urethral surgery,and TGF-β1 has a key role in the process.