类风湿关节炎患者外周血白细胞介素-23和类风湿因子的表达研究

The study of interleukin-23 and rheumatoid factor expression in peripheral blood of patients with rheumatoid arthritis

目的:检测类风湿关节炎(RA)患者体内白细胞介素(IL)-23和类风湿因子(RF)的表达,探讨IL-23在RA发病中的作用及其与RF的关系,为RA的早期诊断和进一步阐明RA的发病机制提供理论依据。方法:采用逆转录-聚合酶链反应(RT-PCR)检测66例RA患者和44例健康对照者外周血单个核细胞(PBMC)中IL-23p19 mRNA的表达水平,采用酶联免疫吸附试验(ELISA)检测血清IL-23浓度,采用免疫比浊法检测血清RF的浓度。结果:RT-PCR显示RA患者PBMC中IL-23p19 mRNA的表达[(45.2±11.7)%]显著高于健康对照组[(23.8±9.5)%],ELISA检测结果显示RA患者血清IL-23水平[(114.47±42.70)ng/L]显著高于健康对照组[(70.81±28.85)ng/L],差异均有统计学意义(均P<0.05)。RA患者血清IL-23水平与RF含量无明显相关性(r=0.389,P>0.05)。结论:RA患者体内IL-23在基因和蛋白水平均过表达,提示IL-23可能参与了RA的免疫病理过程,在RA的发病中发挥作用。

Objective： To detect the expression of interleukin（IL）-23 and rheumatoid factor（RF） in rheumatoid arthritis（RA） patients and to explore the role of IL-23 in the pathogenesis of RA and its relationship with the RF,for early diagnosis of RA and further clarify the pathogenesis of RA provides theoretical basis.Methods： IL-23p19 mRNA expression in peripheral blood mononuclear cells（PBMCs） of 66 RA patients and 44 healthy controls was detected by reverse transcription-polymerase chain reaction（RT-PCR）,serum IL-23 concentration were measured by enzyme-linked immunosorbent assay（ELISA）,serum RF concentrations were measured by immunoturbidimetry.Results： RT-PCR showed IL-23p19 mRNA expression in PBMC of RA patients [（45.2±11.7）%] was significantly higher than that of healthy controls [（23.8±9.5）%],ELISA results showed IL-23 concentration in serum of RA patients [（114.47±42.70） ng/L] was significantly higher than that in healthy controls [（70.81±28.85） ng/L],the differences were significant（P0.05）,but IL-23 and RF showed no significant correlation（r=0.389,P0.05）.Conclusion： IL-23 in gene and protein levels in RA patients are over-expression,suggesting that IL-23 may be involved in the immune pathogenesis of RA and play the role in the pathogenesis of RA.