山莨菪碱(654-2)对内毒素诱导山羊红细胞膜损伤过程中膜脂流动性的影响

Effects of Anisodamine on Erythrocyte Membrane Fluidity during Endotoxin-induced Membrane Injury in Goats

用1,6-二苯基-1,3,5-己三烯(1,6-diphenyl-1,3,5-hexatriene, DPH)分子荧光偏振技术,研究了内毒素(ET)诱导的山羊红细胞膜(ECM)损伤过程中膜脂的流动性(P)、膜脂区微粘度(-η)、脂双层分子排列有序性系数(A)的变化及山莨菪碱(654-2)对其的影响。结果表明,ET 处理组(Ⅱ组)ECM 的P、-η和A 参数在处理后3、5、7、9、12 h 均显著高于Ⅰ组和Ⅲ组(P< 0.05,P< 0.01);Ⅲ组P、-η和A 参数在处理后3 h 显著低于Ⅰ组(P< 0.01), 5、7 h 高于Ⅰ组(P< 0.05,P < 0.01)。结果显示,ET可使山羊ECM P、-η和A 增加、膜流动性降低,而654-2 能有效地改善ET诱导的这些损伤变化,支持了654-2 具有维护膜结构和功能的理论。

The alteration of erythrocyte membrane fluidity and effects of anisodamine were studied with the fluorescence polarization technique, in which 1,6 diphenyl 1,3,5 hexatriene was used as a fluorescence probe, in goats treated with endotoxin. It was found that after administration of endotoxin, erythrocyte membrane fluorescence polarization(P), microviscocity(-η) and fluorescence anisotropy(A) parameters in E.coli endotoxin treatment group (group Ⅱ) were higher than those in the normal control (group Ⅰ) and E.coli and anisodamine coexposure group (group Ⅲ) (P<0.05, P<0.01), during all experiment. These parameters in group Ⅲ were lower than those in group Ⅰ at 3 h(P<0.01) and higher than those in group Ⅰ at 5 h and 7 h (P<0.05, P<0.01). The findings indicated that the erythrocyte membrane changed from a relative fluid to a relative solid after administration of endotoxin, whereas anisodamine had effects of increasing membrane fluidity on erythrocyte membrane of goats in vivo.