抗神经生长因子抗体对变应性鼻炎大鼠鼻黏膜炎症的影响

Effects of anti-NGF antibody on the nasal mucosa inflammation of experimental allergic rhinitis animal

目的:探讨鼻腔滴入抗神经生长因子抗体对变应性鼻炎大鼠鼻黏膜炎症的影响。方法:将40只SD大鼠随机分成实验组30只和对照组(A组)10只,实验组大鼠采用卵清蛋白致敏和激发制成变应性鼻炎大鼠模型,再将大鼠模型随机分为变应性鼻炎组(B组)、抗神经生长因子抗体处理组(C组)、布地奈德治疗组(D组)3组。各组鼻腔给予卵清蛋白激发,每日1次,共7次。C组在激发之前鼻腔给予抗神经生长因子抗体处理,D组在激发之前鼻腔给予布地奈德处理,B组在激发之前鼻腔内以生理盐水代替药物处理。在末次激发之后,通过行为学观察、鼻黏膜病理切片、鼻腔灌洗液和血清中IL-4的含量等指标来评价抗神经生长因子抗体对变应性鼻炎大鼠鼻黏膜炎症的影响。结果:C组和D组行为学评分低于B组,A组无特殊反应;B组鼻黏膜呈典型变应性鼻炎病理改变,可见较多嗜酸粒细胞浸润,鼻腔灌洗液上清液和血清中IL-4的含量均高于A组(P<0.05);C组和D组动物的鼻黏膜病理性改变减轻,嗜酸粒细胞浸润减少,鼻腔灌洗液上清液和血清中IL-4的含量较B组下降明显(P<0.05)。结论:鼻腔滴入抗神经生长因子抗体可有效缓解变应性鼻炎大鼠鼻腔局部症状,减轻鼻黏膜病理性改变,并减少大鼠IL-4的合成与分泌。

Objective： To investigate the effects of anti-NGF antibody on the nasal mucosa inflammation of rat models with allergic rhinitis.Methods： Forty SD rats were randomly divided into 2 groups：the control group（group A） and the experimental group.First of all,those rats in experimental group were sensitized by using ovalbumin（OVA） intraperitoneal immunization and nasal antigen challenge.Group A was treated as a negative control group sensitized and challenged by normal saline instead of OVA.Then,the experimental group was randomly divided into 3 groups： allergic rhinitis（AR） group（group B） was challenged by OVA,anti-NGF antibody group（group C） was challenged by OVA,followed by the administration of anti-NGF antibody,budesonide group（group D） was challenged by OVA,followed by the administration of budesonide.After the last OVA challenge,the level of IL-4 in nasal lavage fluid（NLF） and blood serum were measured by enzyme-linked immunosorbent assay（ELISA）.Results： The symptoms of 8 group B satisfied the diagnosis of AR,no obvious symptoms appeared in group C or group D;Pathological analyses showed significant eosinophil infiltration in the nasal mucosa of group B compared with group C.Compared with group B,the concentrations of IL-4 in NLF and serum of group C decreased significantly（P0.05）.Conclusion： Anti-NGF antibody administered intranasally is effective in treating AR.