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DNA-PK蛋白功能及其调控机制 被引量：3

DNA-PK Function and Regulatory Mechanism
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摘要 DNA依赖性蛋白激酶（DNA-dependent protein kinase,DNA-PK）是由3个亚基组成的丝/苏氨酸蛋白激酶,属于磷脂酰肌醇-3激酶相关激酶家族（phosphatidylinositol 3-kinase-related kinases,PIKK）,是基因组DNA损伤修复过程中的关键蛋白激酶,参与并决定着非同源末端连接DNA损伤修复通路的整个进程.此外DNA-PK还参与了电离辐射诱导的凋亡信号转导通路,免疫细胞V（D）J重组、免疫细胞分化、胰岛素刺激下的细胞应答等过程,具有维持端粒稳定性的功能.DNA-PK活性的升高会降低肿瘤对放射的敏感性,其活性主要受自身磷酸化调控,此外活性氧、EGFR、MG132抑制剂、PP1γ1和PP5等蛋白磷酸酶也有调控DNA-PK活性的作用. DNA-dependent protein kinase （ DNA-PK）, a serine/threonine kinase consisting of three subunits, is a member of the PIKKs （phosphoinositide 3-kinase-like family of protein kinases） . DNA-PK is the key protein kinase in the genomic DNA damage repair, participating in and determining the whole process of non-homologous end-joining. Furthermore, DNA-PK is involved in ionizing radiation stimulated apoptotie signal transduction, V （D） J recombination, immune cell differentiation, insulin-stimulated cellular responses, and maintenance of telomere stabili- ty. Increase of DNA-PK activity reduces the sensitivity of tumors to radiation. The activity of DNA-PK is mainly regulated by its autophosphorylation. Reactive oxygen species, EGFR, MG132 inhibitor, and protein phosphatase such as PP1 T1 PP5 also play a role in control of DNA-PK activity.
作者 江娜 沈瑛 孔宪明 糜军
机构地区 上海交通大学医学院医学科学研究院
出处 《医学分子生物学杂志》 CAS CSCD 2011年第3期278-282,共5页 Journal of Medical Molecular Biology
关键词 DNA依赖性蛋白激酶 激酶相关激酶家族 蛋白异质二聚体 DNA损伤修复 DNA-PK PIKK Ku70 Ku80 DNA repair
分类号 R345 [医药卫生—基础医学]
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参考文献39

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同被引文献34

  • 1高国兰,万红英,黄传生,邹学森,陈文学.P-gp、GST-π、TS蛋白在宫颈癌中的表达及临床意义[J].临床肿瘤学杂志,2007,12(1):23-26. 被引量：11
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医学分子生物学杂志
2011年 第3期

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