西花蓟马对多杀菌素的抗性汰选和遗传方式

Laboratory selection and inheritance mode of resistance to spinosad in the western flower thrips,Frankliniella occidentalis

在室内进行了西花蓟马Frankliniella occidentalis(Pergande)种群对多杀菌素的抗性筛选和抗性遗传方式分析。经过2年多的汰选,抗性水平相比较初始种群提高了30倍,与同时测定的敏感品系相比抗性达到80.8倍。抗性遗传方式分析结果表明,正、反交后代显性度分别为0.51和0.43,二者差异不显著,说明西花蓟马对多杀菌素的抗性为常染色体、不完全显性遗传;剂量对数死亡机率值曲线分析结果显示回交后代在死亡率50%处,自交后代在死亡率25%和75%处未出现明显平坡,回交和自交后代实测值卡方检验进一步证实抗性为多基因控制。

The response of western flower thrips,Frankliniella occidentalis （Pergande）,to selection for resistance to spinosad under laboratory conditions,and the mode of inheritance of spinosad resistance was investigated.After more than 2 years of selection resistance to spinosad in the selected strain increased 30-fold compared to the original strain,and 80.8-fold compared to the susceptible strain.The progeny of reciprocal F1 crosses （resistant male×susceptible female and resistant female×susceptible male） responded similarly in bioassays,with values of 0.51 and 0.42 for D（degree of dominance）,respectively,indicating that resistance was autosomal and incompletely dominant.Analysis of dose-response lines indicates that there were no plateaus at 50% mortality of backcross progeny （F1×susceptible strain） and at 25% and 75% mortalities of self-cross of backcross progeny.Chi-square analysis of the resistance of progeny from backcrosses （F1×susceptible strain） and self-cross of backcrosses suggest that resistance to spinosad is probably controlled by several genes.