核基质附着区结合蛋白与皮肤鳞状细胞癌的相关性研究

Relationship between matrix attachment region-binding proteins and skin squamous cell carcinoma

目的探讨核基质附着区结合蛋白（MARBP）与皮肤鳞状细胞癌（SCC）的相关性，筛选相关性较高的MARBP。方法以取材自2例SCC患者正常皮肤组织和HaCaT细胞作为对照，应用RT—PCR方法，检测部分已知与肿瘤相关的MARBP（CUX1、LaminB1、Nucleolin、hnRNPU、SAFB2、SATB1、YBX1）mRNA在12例SCC肿瘤组织和瘤旁组织、人皮肤鳞状细胞癌细胞系Colo-16细胞、人表皮癌A431细胞的表达水平。结果所有被检测的MARBPmRNA在2例正常皮肤组织均未见明显表达，在12例SCC肿瘤组织和瘤旁组织、Colo-16、A431细胞系中均有不同程度和不同比例的表达，在HaCaT细胞系中，除SATB1外，其余MARBPmRNA均有表达。SCC肿瘤组织和瘤旁组织MARBPmRNA表达水平的比较显示CUX1、Nucleolin和YBX1差异有统计学意义；LaminB1、hnRNPU、SAFB2和SATB1差异无统计学意义。结论CUXl、Nucleolin和YBX1等MARBPrnRNA可能参与SCC的发生、发展；

Objective To estimate the relationship between matrix attachment region-binding proteins （MARBPs） and skin squamous cell carcinoma （SCC）, and to screen for highly-associated MARBPs. Methods Skin specimens were resected from the tumor and tumor-adjacent normal tissue of 12 patients with SCC. Normal skin tissue from the abdomen of 2 of the 12 patients served as the control. Reverse-transcription PCR was per- formed to detect the mRNA expressions of seven MARBPs, including CUX1, Lamin B1, Nucleolin, hnRNP U, SAFB2, SATB1 and YBX1, in these specimens and in a human keratinocyte cell line HaCaT and two cell lines Colo-16 and A431 derived from human SCC. Results No evident expression was observed for the seven MARBPs in the normal control specimens, while the MARBPs were expressed in the tumor tissue and tumoradjacent tissue specimens, Colo-16 cells and A431 cells at different degrees and frequencies. In HaCaT cells, all the 7 MARBPs except SATB1 were expressed. Statistical analysis showed a significant difference in the expression of CUX1, Nucleolin and YBX1, but not in that of Lamin B1, hnRNP U, SAFB2 or SATB1 between the tumor and tumor-adjacent normal tissues. Conclusion Some MARBPs such as CUX1, Nucleolin and YBX1 may participate in the development of skin squamous cell carcinoma.