E选择素和P选择素在结节性血管炎皮损中的表达

Expression of E-selectin and P-selectin in nodular vasculitis lesions

目的探讨E选择素、P选择素在结节性血管炎中的意义。方法分别检测70例结节性血管炎及24例正常皮肤组织中E选择素、P选择素的表达情况，比较两组间E选择素、P选择素表达的差异以及在结节性血管炎中两者之间的相互关系。结果70例结节性血管炎E选择素表达均为阳性，以中度阳性（＋＋）为主；24例正常皮肤组织均为阴性；两组比较，差异有统计学意义（P〈0．01）。70例结节性血管炎P选择素表达均为阳性，以强阳性（＋＋＋）为主；24例正常皮肤P选择素表达仅有2例弱阳性，其余为阴性，两组比较，差异有统计学意义（P〈0．01）。结节性血管炎中E选择素组与P选择素组比较，两者表达相关性好（P〈0．01）。对患者年龄、性别比较，E选择素、P选择素表达无统计学差异（P〉0．05）。结论E选择素、P选择素的表达与结节性血管炎有一定的相关性，且在急性期两者相互关联。

Objective To estimate the significance of E-selectin and P-selectin in nodular vasculitis. Methods The EnVision two-step method was used to measure the expression of E-selectin and P-selectin in skin samples from the lesions of 70 patients with nodular vasculitis and normal skin of 24 human controls. The differences between the patients and controls in the expression of E-selectin and P-selectin and relationship between their expression levels in nodular vasculitis lesions were assessed. Results The expression of E-selectin was detected in all the specimens of nodular vasculitis, and most of the expression level was moderately intensive （＋＋）; while E-selectin was absent in all of the control specimens. All the specimens of nodular vasculitis stained postivive for P-selectin, which was strongly （＋＋＋） expressed in most of the specimens; while only 2 con- trol specimens stained weakly positive for P-selectin. A significant difference was observed in the expression of E-selectin and P-selectin between the specimens from the patients and controls （both P 〈 0.01 ）, but not among specimens from patients at different ages and between specimens from female and male patients （all P 〉 0.05 ）. In addition, the expression of E-selectin and P-selectin was well correlated with each other in lesions of nodular vasculitis （P 〈 0.01 ）. Conclusions The expression of E-selectin and P-selectin is correlated with each other in lesions of acute nodular vasculitis, and is associated with the development of nodular vasculitis.