葫芦种子传黄瓜绿斑驳花叶病毒的检测

Detection of the Cucumber green mottle mosaic virus in seeds of bottle gourd

从感染黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的葫芦植株上收取种子,通过苗期症状观察法、双抗体夹心酶联免疫吸附法(DAS-ELISA)、免疫捕获反转录PCR(IC-RT-PCR)法测定葫芦种子的带毒情况,并用生物学接种方法测定葫芦种子携带病毒的侵染活性。苗期症状观察法结果表明,199株幼苗有2株表现花叶斑驳症状,种子传毒率为1.01%;而利用DAS-ELISA和IC-RT-PCR法随机检测30粒葫芦病株种子,CGMMV检出率为100%。种子各部位携带的CGMMV接种葫芦表现典型的花叶斑驳症状,表明葫芦种子携带的CGMMV具有侵染活性。DAS-ELISA检测葫芦种子CGMMV的灵敏度为1/5120种子研磨液。

The seed transmission rate of Cucumber green mottle mosaic virus （CGMMV） in the bottle gourd Lagenaria siceraria was detected based on seedling symptoms, DASELISA and immunocapture RTPCR （ICRTPCR）. Seed transmission ratio of CGMMV in bottle gourd from the viruscontaminated seeds was 1.01%, while seedborne ratio of CGMMV in bottle gourd seeds tested was 100% by DASELISA and ICRTPCR. CGMMV in bottle gourd seeds was demonstrated to have infection activity by biological inoculation. The sensitivity of DASELISA used for detection of CGMMV in the seed sap of bottle gourd was 1/5120.