摘要
本文根据质量平衡原理建立了反相色谱双保留机理的质量平衡模型,导出了色谱流出曲线的一阶矩和二、三阶中心矩,获得了双保留机理色谱容量因子和塔片高度的表达式.讨论了各种色谱动力学参数对柱效和峰形的影响,对改善色谱峰对称度和提高柱效的措施作了理论说明。
The mass balance model of daul retention mechanism in RP-HPLC is establishedbased on the theory of mass transfer. The first moment and the second, third cen-tral moments are derived. The total capacity factor k' and HETP in daul retentio-mechanism can be expressed as: The effects of chromatographic parameters on column efficiency and peak shape arediscussed. The approaches to increase column efficiency and eliminate the tail ofchromatogram are explained.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1990年第3期283-289,共7页
Acta Physico-Chimica Sinica
