胚胎干细胞抗Lewis非小细胞肺癌作用的体内研究

Embryonic stem cells against Lewis non-small cell lung cancer in vivo

目的研究胚胎干细胞动物体内抗Lewis非小细胞肺癌的作用及初步机制。方法以小鼠Lewis肺癌模型为基础，检测实验组和对照组小鼠的肿瘤生长情况和免疫指标，探讨胚胎于细胞在体内的抗肿瘤作用及其初步分子免疫机制。结果肿瘤生长曲线显示，与对照组小鼠相比，实验组小鼠肿瘤的生长速度明显减慢，从实验的第5天开始两组小鼠的肿瘤体积相比差异均有统计学意义（P〈0．05）。小鼠外周血T、B淋巴细胞亚群检测结果，与对照组小鼠相比，实验组小鼠外周血CD4＋、CD8＋淋巴细胞比例增加，差异有统计学意义，分别为P〈0．05、P〈0．01，CD19＋淋巴细胞比例下降（P〈0．05）。血清细胞因子（IL-2、IL-4和IFN-γ）检测结果，与对照组相比，实验组小鼠外周血中IL-2和IFN-γ细胞因子水平增高，差异有统计学意义（P〈0．05），IL-4略有增高但无统计学意义。结论胚胎干细胞不仅在体外具有明显的抗肿瘤作用，在动物体内胚胎干细胞也能通过激活免疫系统从而产生有效的抗肿瘤作用，抑制或延缓肿瘤的形成和发展。

Objective To investigate the function and mechanism of embryonic stem cells against Lewis non-small cell lung cancer in vivo. Methods Based on the mouse Lewis non-small cell lung cancer model, we have tested some tumor growth indexes and investigated the immune response of embryonic stem cells against cancer cells. Results Compared with the mice in control group, mice in experimental group received obvious antitumor immunity, which means more activated lympbocytes and antitumor cytokines, resulted in the effective control and inhibition of tumor development. Conclusion Besides the antitumor effect in vitro, embryonic stem cells can also generates immune response in vivo, which could effectively inhibit and/or delay the development of cancer.