金钗石斛生物总碱对脂多糖激活星形胶质细胞产生炎症因子的影响

Effects of Dendrobium nobile total alkaloids on lipopolysaccharide-induced astrocyte activation and pro-inflammatory factors production

目的观察金钗石斛生物总碱对外源性内毒素脂多糖(lipopolysaccharide,LPS)激活大鼠大脑皮层星形胶质细胞(astrocyte)及诱导其产生和释放炎症介质的影响,探讨石斛生物总碱对星形胶质细胞的抗炎作用。方法通过MTS检测细胞存活率,ELISA法检测TNF-α炎性因子蛋白的表达,实时定量多聚酶链反应(real time RT-PCR)检测炎症相关基因TNF-α、IL-6 mRNA的表达。结果①LPS刺激星形胶质细胞后,MTS检测吸光度明显升高,与正常组比较差异有显著性;②金钗石斛生物总碱能够降低LPS所致的TNF-α蛋白的高表达(P<0.05);③金钗石斛生物总碱能够降低LPS诱导的星形胶质细胞吸光度的升高,同时明显抑制LPS所致的TNF-α、IL-6 mRNA的高表达。结论金钗石斛生物总碱能够拮抗LPS所引起的炎症反应,其作用与抑制星形胶质细胞的激活及其炎症因子的释放密切相关。

Aim To investigate the effects of Dendrobium nobile total alkaloids on lipopolysaccharide（LPS）-induced activation of astrocytes and the subsequent release of pro-inflammatory factors.Methods The cell viability was tested by the MTS assay.The generation and release of TNF-α from astrocytes were evaluated via the enzyme link immunosorbent assay（ELISA）.The mRNA expressions of TNF-α,IL-6 in astrocytes were detected by real-time RT-PCR.Results①LPS activated astrocytes resulting in significantly elevated absorbance was tested by the MTS;②Dendrobium nobile total alkaloids inhibited the LPS-induced over expressions of TNF-αprotein（compared with control group P0.05）;③Compared with the LPS group,Dendrobium nobile total alkaloids significantly inhibited LPS-induced activation of astrocytes and decreased LPS-induced mRNA overexpressions of TNF-αand IL-6.Conclusion Dendrobium nobile total alkaloids can protect against LPS-induced neuroinflammation.This neuroprotection is partly due to the inhibition of astrocyte activation and the reduced production of pro-in-flammatory factors.