海洋细菌822发酵液中挥发性成分GC/MS分析

Analysis of the volatile composition of fermental liquid from marine-borned bacterium 822 by GC/MS

采用气相色谱质谱联用(GC/MS)分析用乙酸乙酯从海洋细菌822发酵液中萃取得到的挥发性成分,质量分数用面积归一化法计算。结果表明,该菌18个峰产生的15种挥发性物质主要有烷类、酮类、醇类、酸类和酯类,其中5种主要成分总含量达到98.38%,分别是2,3-丁二醇、3-甲基丁酸、2-甲基丁酸、2-甲戊酸酯和2-甲丙酸戊酯,含量分别为77.06%、5.00%、5.46%、6.80%和4.06%,是海洋细菌822的主要代谢产物。

Volatile of fermental liquid of marine-derived bacterium 822 was extracted by ethyl acetate gas chromatography-mass spectrometry （GC-MS） to determine its chemical component,and normalization was employed to deter-mine its relative content. Results showed that 18 peaks and 15 compounds given by GC-MS was identified, the main five kinds components of 15 compounds which contained silane, ketone, alcohols, acids and esters reached about 98.38%. The five kinds components were 2,3 - butyl glycol, 3-methyl butyrate, 2-methyl butyrate, 2-butanoice acid ester, 2-methyl propionic acid ester, and the percentages content were 77.06, 5.46, 5.00, 6.80 and 4.06 respectively. They were metabolites of marine bacterium 822.