京尼平与戊二醛鞣制牛心包材料的对比研究

Comparison of genipin-and glutaraldehyde-tanned bovine pericardia

目的观察京尼平作为一种新型固定生物瓣膜组织交联剂的可行性。方法牛心包经脱细胞处理,分别用戊二醛和京尼平进行鞣制,光镜下观察脱细胞效果,电镜下观察胶原纤维排列;测试收缩温度;拉伸试验测定最大载荷;Wistar大鼠臀大肌皮下埋藏牛心包片,于4周及8周后取出,分别测试京尼平与戊二醛鞣制的牛心包的钙、磷含量。结果经脱细胞处理后,光镜下可见脱细胞处理的牛心包几乎没有细胞核,而未经脱细胞处理的牛心包可见弥漫存在的细胞核。电镜下可见京尼平鞣制的牛心包胶原纤维呈环状交联,戊二醛鞣制的牛心包胶原纤维呈网状交联,京尼平鞣制的牛心包胶原纤维排列较戊二醛鞣制的牛心包略为疏松,收缩温度略低于戊二醛鞣制的牛心包(P<0.05)。最大载荷无统计学差异(P>0.05)。但京尼平鞣制牛心包的钙、磷含量显著低于戊二醛鞣制的牛心包(P<0.05)。京尼平鞣制的牛心包8周的钙含量显著低于4周的钙含量(P<0.05)。结论京尼平鞣制的牛心包具有较高的强度和极低的钙、磷含量,且随时间延长京尼平鞣制的牛心包的钙含量有减少趋势,提示京尼平是一种很有前景的生物组织交联试剂。

Objective To evaluate the feasibility of genipin as a new biological valve cross-linking agent.Methods Acellular bovine pericardia were respectively treated with glutaradehyde and genipin for tanning.The HE slides of bovine pericardial were observed under a light microscope to identify the decellularized effect.Collagen fiber arrangements were observed under an electron microscope.Shrinkage temperature and tensile strength of the biological materials were tested.Bovine pericardia were buried in the subcutaneous of the musculus glutaeus maximus of Wistar rats for 4 weeks or 8 weeks,and then calcium and phosphorus contents were determined in them.Results In decellularized bovine pericardial slides,there was almost no nucleus,while in non-decellularized ones,there were many nuclei.Collagen fibers formed a ring in genipin-tanned bovine pericardial tissues,while they were reticulated in glutaraldehyde-tanned tissues.Collagen fiber arrangements were a little looser in genipin tanning than in glutaraldehyde tanning.The shrinkage temperature of genipin-tanned tissues was lower than that of glutaraldehyde-tanned ones（P〈0.05）.The difference had no statistical significance in maximum tensile strength between genipin tanning and glutaraldehyde tanning（P〉0.05）.Calcium and phosphorus contents in genipin tanning were significantly lower than those in glutaraldehyde tanning at 4 weeks and 8 weeks（P〈0.05）.The calcium content in genipin tanning was significantly lower at 8 weeks than at 4 weeks （P〈0.05）.Conclusion Calcium and phosphorus contents of genipin-tanned bovine pericardial tissues are significantly lower than those of glutaraldehyde-tanned ones,and the calcium content in genipin-tanned bovine pericardial tissues decreased with time.Tensile strength is higher in genipin-tanned bovine pericardial tissues.Consequently,genipin is a promising cross-linking agent for biological tissues.