白细胞介素-17对哮喘小鼠嗜酸性粒细胞的影响

Effects of interleukin-17 on eosinophils in mice with asthma

目的探讨白细胞介素-17(IL-17)及其抗体对哮喘小鼠肺中嗜酸性粒细胞(EO S)的影响。方法随机将60只Balb/c雌性小鼠分为健康组(A)、对照组(B)、哮喘组(C)、IL-17抗体组(D)及IL-17组(E)各12只。A组不做特殊处理,B、C、D、E组采用卵蛋白致敏和激发的方法,建立哮喘模型并给予相应干预;采用HE染色肺组织病理切片观察炎性细胞浸润,采用ELISA法检测小鼠肺组织匀浆上清液中嗜酸性粒细胞趋化因子(Eotax in-1)。结果 C、D、E组小鼠气道炎症以EO S和中性粒细胞浸润为主,B组变化较轻,A组正常;Eotax in-1浓度从高到低依次为C、D、E、B、A组(P<0.05),其差异有统计学意义。结论外源性IL-17能够减少激发状态下哮喘小鼠肺中EO S的浸润。

Objective To investigate effects of interleukin-17（IL-17） and the IL-17 neutralization antibody on eosnophils and eotaxin-1 in asthma mice.Methods A total of 60 Babl/c female mice were randomly divided into five groups ： the healthy group（group A）,the control group（group B）,the asthma group（group C）,the IL-17 neutralization antibody group（group D） and the IL-17 group（group E）,12 mice in each group.Mice in group B,C,D and E were sensitized and challenged with ovalbumin to establish asthma models.Pathological characteristics of lung tissues were observed using HE staining.Eotaxin-1 in mouse lung tissue homogenate was determined by ELISA.Group A was not treated with particular methods.Results Airway inflammation in asthmatic mice was mainly characterized by eosinophil and neutrophil infiltration,which was less observed in the normal control group.Concentrations of eotaxin-1 in lung tissue homogenate in group C,D,E,B and A were in the order from high to low.The difference between the five groups was significant（P〈0.05）.Conclusion Exogenous IL-17 may inhibit infiltration of eosinophils in the lung of asthma mice.