云南双江佤族、布朗族15个STR基因座遗传多态性及遗传关系分析

Polymorphism and genetic relationship of 15 STR loci in Wa and Bulang populations in Shuangjiang County,Yunnan Province

目的检测云南双江县佤族、布朗族15个STR基因座遗传多态性及与其他群体的遗传关系。方法以Power PLex16system Kit复合扩增15个STR基因座(D3S1358、THO1、D21S11、D18S51、Penta E、D5S818、D13S317、D7S820、D16S539、CSF1PO、Penta D、VWA、D8S1179、TPOX、FGA),用ABI PRISM310遗传分析仪检测扩增产物,计算群体等位基因频率等法医学参数,并结合文献发表的国内其他群体的遗传学资料,运用CODIS系统13个基因座计算群体间的遗传距离。结果 15个STR基因座在佤族、布朗族中均具有较高的遗传多态性,适于群体的法医学应用。群体遗传距离比较中,佤族与布朗族最近(0.011 0),佤族与布依族及鄂温克族分别为0.030 2和0.024 3;佤族与汉族群体的遗传距离普遍较近,最短为浙江汉族(0.014 0)。布朗族与布依族、鄂温克族遗传距离分别为0.034 3和0.030 9,与汉族群体的距离也较近,最短为安徽汉族(0.012 4)。结论 15个STR基因座在云南双江佤族、布朗族中具有较高的遗传多态性,适于群体的法医学应用;群体分析对了解佤族、布朗族群体的起源、迁徙及其遗传关系有重要意义。

Objective To investigate genetic polymorphisms in Wa and Bulang populations in Shuangjiang County, Yunnan Province, and to evaluate their forensic application values and genetic relationships among other populations.Methods Fifteen STR loci included in the Power PLex 16 system Kit were amplified. The PCR products were analyzed by ABI Prism 3100 Genetic Analyzer. Forensic parameters of the two minorities were obtained, and genetic distances between Wa,Bulang and other populations were calculated by STR loci included in the CODIS system. Results In Wa and Bulang populations, the 15 STR loci were polymorphic.In genetic distance analysis, the distance between Wa and Bulang was the nearest（0.011 0）.The other distances were as follows： 0.030 2,between Wa and Buryat;0.024 3,between Wa and Ewenke;0.034 3,between Bulang and Buryat;and 0.030 9,between Bulang and Ewenke.The genetic distances of Wa and Bulang with Han populations were both near.The distance was 0.014 0 between Wa and Han populations in Zhengjiang Province, and 0.012 4 between Bulang and Han populations in Anhui Province.Conclusions The 15 STR loci were polymorphic in Wa and Bulang, which could provide a powerful discrimination tool for forensic applications.The investigations of genetic distances between different populations are useful to study their origins, migrations and genetic relationships.