WCX纳米磁珠在子宫内膜癌血清蛋白质组中的应用

Application of WCX magnetic beads to serum proteome profiling in endometrial carcinoma

目的利用弱阳离子交换型（WCX）纳米磁珠联合表面增强激光解析离子化飞行时间质谱（SELDI-TOF-MS）分析子宫内膜癌患者血清蛋白质谱,筛选潜在的特异性肿瘤标志物,探讨其临床意义。方法应用WCX纳米磁珠联合SELDI-TOF-MS对24例子宫内膜癌患者及28例正常人血清标本进行蛋白质质谱分析,用BiomarkWizard软件分析差异蛋白峰,并在Swiss蛋白数据库中搜索鉴定差异蛋白,受试者工作特征（ROC）曲线评价该技术的应用价值。结果在质荷比（m/z）2 00020 000范围内,子宫内膜癌患者血清中筛选出与正常人血清有统计学意义的差异蛋白11个（P〈0.05）,其中6个高表达：m/z分别为4 175、5 315、7 970、8 047、15 930、16 074;5个低表达：m/z分别为4 592、4 675、7 019、9 188、9 358。m/z为7 970的差异蛋白与子宫珠蛋白（UG）相符。应用ROC曲线分析,确定11个差异蛋白对子宫内膜癌有中等诊断价值,灵敏度为70.5%96.4%,特异度为89.3%100%。结论 WCX纳米磁珠联合SELDI-TOF-MS能在子宫内膜癌患者血清中筛选出灵敏度和特异度较好的差异表达蛋白,其有助于子宫内膜癌的筛查、早期诊断及预后监测。

Objective To explore the serum proteomic spectrum in endometrial carcinoma,screen for its potential special biomarkers by weak cation exchange（WCX） magnetic beads and surface-enhanced laser desorption/inionation-time of flight-mass spectrometry（SELDI-TOF-MS）,and evaluate its clinical significance.Methods The proteomic spectrum was analyzed in 24 endometrial carcinoma patients and 28 healthy controls by WCX magnetic beads coupled with SELDI-TOF-MS.Differently expressed proteins were detected by Biomark Wizard software,and searched for in the Swiss protein database.The clinical value was evaluated by a receive operating characteristic（ROC） curve.Results A diagnostic pattern consisting of 11 differential protein peaks was established.In a range of mass-to-charge ratio（m/z） 2 000-20 000,11 statistically differently expressed proteins were identified in the serum of patients with endometrial neoplasm compared with the healthy controls,including 6 up-regulated proteins with m/z of 4 175,5 315,7 970,8 047,15 930 and 16 074 and 5 down-regulated ones with m/z of 4 592,4 675,7 019,9 188 and 9 358.Uteroglobin accorded with the differently expressed protein with an m/z of 7 970 in the Swiss protein database.The sensitivity andspecificity of 11 differently expressed proteins in the diagnosis of endometrial carcinoma were 70.5%-96.4% and 89.3%-100%,respectively.Conclusion WCX magnetic beads coupled with SELDI-TOF-MS could screen for potential protein biomarkers with high sensitivity and specificity in the serum of endometrial carcinoma,and it is a helpful tool for the screening,early diagnosing and prognostic monitoring of endometrial carcinoma.