腺周肌纤维母细胞标志物在胃癌及癌前病变中的表达

Marker expression of pericryptal myofibroblasts in gastric carcinoma and precancerous lesions

目的探讨在胃黏膜癌变演进过程中,间质肌纤维母细胞标志物α-平滑肌肌动蛋白(α-SMA)的表达变化及其与增殖细胞核抗原(PCNA)的关系。方法采用免疫组化方法,检测胃癌及癌前病变中α-SMA和PCNA表达,并分析它们的相关性。结果α-SMA表达在肠化生黏膜中升高,低、高级上皮内瘤变表达较肠化黏膜降低,在胃癌中表达再次升高(χ2=56.15,P<0.01)。α-SMA的分布模式亦有差别,肠化生黏膜呈腺周表达模式,向胃癌进展过程中腺周表达模式逐渐弱化。PCNA表达随胃黏膜癌变演进状态的改变逐渐增高(χ2=117.12,P<0.01)。α-SMA与PCNA表达呈显著正相关(rs=0.61,P<0.01)。结论间质肌纤维母细胞在胃黏膜癌变演进过程中表达强度及分布均存在明显变化,提示其功能具有异质性,参与了胃癌的恶性转化过程。

Objective To investigate expression change of alpha-smooth muscle actin（α-SMA）,the marker of pericryptal myofibroblasts,in gastric carcinoma and precancerous lesions,and study its relationship with the proliferating cell nuclear antigen（PCNA）.Methods The immunohistochemical technique was used to detect expression of α-SMA and PCNA in gastric carcinoma and precancerous lesions.The relationship between α-SMA and PCNA was analyzed.Results Expression of α-SMA was increased in intestinal metaplasia,then decreased in low or high grade gastric intraepithelial neoplasia,and increased again in gastric carcinoma.（χ2=56.15,P〈0.01）.The pericryptal distribution of α-SMA was found in intestinal metaplasia,but diminished along with the development of gastric carcinoma.Expression of PCNA was increased along with the development of gastric carcinogenesis（χ2=117.12,P〈0.01）.There was a positive correlation between α-SMA and PCNA expressions（rs =0.61,P〈0.01）.Conclusions There are significanct changes in expression intensity and distribution of stroma myofibroblasts with the development of gastric carcinoma,which indicates that myofibroblasts might play a role in gastric carcinogenesis.