大白菜中硫代葡萄糖苷的鉴定及含量分析

Glucosinolate Profile and Accumulation in Brassica campestris L. ssp. pekinensis

以129份大白菜[Brassca campestris L.ssp.pekinensis(Lour.)Olsson]DH系为材料,采用HPLC法,对不同季节生长的大白菜叶片中硫苷的组分和含量进行了研究。在129份材料中均检测到8种硫苷成分,包含3种脂肪族硫苷,4种吲哚族硫苷和1种芳香族硫苷。主成分分析表明脂肪族硫苷3-丁烯基硫苷(NAP)、4-戊烯基硫苷(GBN)和2-羟基-3-丁烯基硫苷(PRO)是大白菜硫苷的主要组分,占总硫苷含量的60%。筛选出了6份高硫苷含量的材料。联合方差分析结果表明,脂肪族硫苷NAP、GBN和PRO在不同品种间差异极显著;某些吲哚族硫苷受环境影响较大,在不同的季节间差异显著;芳香族硫苷2-苯乙基硫苷(NAS)的含量在季节和季节×品种间差异显著。不同硫苷间的积累存在相互影响,NAP与GBN、NAP和GBN与总硫苷在两个年份中均呈极显著正相关。

The 129 Brassica campestris L. ssp. pekinensis（Lour.）Olsson DH lines grown in different seasons were used to identify glucosinolate profile and accumulation in leaves by HPLC analysis. Eight main glucosinolates were detected in all the accessions including 3 aliphatic glucosinolates,4 indolic glucosinolates and 1 aromatic glucosinolate. Principal component analysis showed Aliphatic glucosinolates,Gluconapin（NAP）,Glucobrassicanapin（GBN）and Progoitrin（PRO）were the main glucosinolate profiles with the highest ratio of about 60.0% in Chinese cabbage,and 6 materials with a high content of glucosinolate were identified. Combined variance analysis showed that there were significant differences among varieties in aliphatic glucosinolates（NAP,GBN and PRO）. The accumulation of several indolic glucosinolates was affected greatly by environment,and a significant variance was appeared in different seasons. The aromatic glucosinolate,Gluconasturtiin（NAS）concentration varied significantly between two different seasons and season × variety interaction.Correlations analysis between individual glucosinolates showed that the accumulation of different glucosinolates was affected by each other,and correlation between NAP and total glucosinolate content,GBN and total glucosinolate,NAP and GBN was all significant respectively in the two seasons.