参附注射液对心肌缺血/再灌注损伤血流动力学和心肌酶的影响

The protective of Shenfu injections on hemodynamics and myocardial enzyme after myocardial ischemia/reperfusion injury

目的:探讨参附注射液对家兔心肌缺血/再灌注(I/R)损伤的保护作用及其机制。方法:家兔30只,随机分为3组(n=10):对照组、心肌缺血/再灌注损伤(MI/RI)组和参附注射液组,统一标准喂养。行药物预处理10 min后,手术结扎家兔冠状动脉左心室支,建立急性心肌缺血再灌注模型,观察急性心肌缺血和再灌注状态下血流动力学及心肌组织中酶的变化。结果:与对照组比较:MI/RI组心脏舒缩功能减退,丙二醛(MDA)含量增高,超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)和细胞能源Na+-K+-ATP酶及Ca2+-ATP酶活性降低,乳酸脱氢酶(LDH)、肌酸激酶(CK)大量释放。而与MI/RI组比较:参附注射液组能不同程度的恢复左心室收缩压(LVSP)、心室内压最大变化速率(±dp/dtmax)(P<0.01),降低左室舒张末期压(LVEDP)(P<0.01),抑制MDA、LDH、CK升高,增强SOD、GSH-PX、Na+,K+-ATP及Ca2+-ATP活力。结论:参附注射液对心肌缺血/再灌注损伤具有明显的保护作用。

Objective：To study the protective effect of Shenfu（Chinese traditional medicine） injections on myocardial ischemia/reperfusion（I/R） injury in rabbits.Methods：Thirty rabbits were divided into three groups（n=10） randomly：control group,myocardial ischemia/reperfusion group（MI/RI） and Shenfu injections extract group,three groups of rabbits were fed respectively with standard diet.After giving medicine 10 minutes,the myocardial ischemia/reperfusion injury animal model was established by ligaturing rabbits left ventricutar branch of coronary artery,and observing the changes of enzyme/hemodynamics during myocardial ischemia/reperfusion.Results：In model group myocardial function of shrink went down,the amount of malondialdehyde（MDA） was higher,the activity of superoxide dismutase（SOD）,glutathine peroxidease（GSH-PX）,Na＋-K＋-ATP and Ca2＋-ATP were lower,lactic dehydrogenase（LDH） and creatine kinase（CK） were released compared with those in model group.Shenfu injections could recover left ventricular systolic pressure（LVSP） and±dp/dtmax,decrease left ventricular end-diastolic pressure（LVEDP）,inhibit the increasing of MDA,LDH and CK,and increase the activaty of SOD,GSH-PX,Na＋-K＋-ATP and Ca2＋-ATP.Conclusion：Shenfu injections can obviously protect myocardial ischemia/reperfusion injury.