高原脑水肿患者血浆的蛋白质组学研究

The study of plasma proteomic changes in a patient with high-altitude cerebral edema

目的:高原脑水肿是急性高原病的重症表现之一,其发病机制尚不完全清楚。本实验目的是应用蛋白质组学方法研究高原脑水肿发病时血浆蛋白质表达的变化,为研究高原脑水肿发病分子机制以及高原脑水肿的预防、诊断及治疗提供重要的分子标志物。方法:应用二维凝胶电泳(2D)结合质谱的方法比较了一例高原脑水肿(HACE)患者与高原肺水肿(HAPE)和轻型急性高原反应(mAMS)患者血浆蛋白表达的差异,对差异蛋白进行鉴定,最后用ELISA方法检测载脂蛋白E含量。结果:我们检测到HACE与HAPE患者血浆比较有6个差异蛋白质点,HACE与mAMS患者血浆比较也有6个差异蛋白质点,质谱鉴定结果显示两组比较中都有载脂蛋白E含量变化,ELISA检测结果与二维凝胶电泳结果一致。结论:本实验首次将蛋白质组学技术应用到高原脑水肿研究中,并且发现了可能与高原脑水肿发病密切相关的载脂蛋白E,该蛋白对于研究高原脑水肿发病分子机制以及疾病的预防、诊断及治疗的意义有待进一步研究。

Objective：High-altitude cerebral edema（HACE） is one of the most serious acute mountain sickness and its underlying mechanism is still unknown clearly.The aim of this study was to determine the changes of plasma proteins in high altitude cerebral edema patients for discovering essential biomarkers used for the routine prophylaxis,diagnosis and treatment.Methods：Plasma protein profiling two dimensional gel electrophoresis followed by mass spectrometry was used to explore protein alterations in one patient with high-altitude cerebral edema（HACE）.Striking differences in two-dimensional gel proteomes of plasma were observed between high-altitude cerebral edema（HACE） and high-altitude pulmonary edema（HAPE） and between high-altitude cerebral edema（HACE） and mild acute mountain sickness（mAMS）.Furthermore,apolipoprotein E altered in high-altitude cerebral edema was validated by ELISA.Results：Different six spots were found in this study from the comparison between HACE and HAPE,and there were different six spots which were detected from the plasma of HACE patient in comparison to mAMS.Apolipoprotein Ewas identified in the two groups of comparative maps and results of ELISA consisted with the results of 2-DE.Conclusion：In this study,we used proteomic approach to explore HACE firstly and found different proteins that were probably associated with HACE.This would offer a clue to a better understanding of HACE for precaution,diagnosis and treatment.