家兔肠系膜上动脉闭塞性休克前后的血清比较蛋白质组学初步研究

Primary study on changes of serum proteomics in rabbit superior mesenteric artery occlusion shock

目的:探讨家兔肠系膜上动脉闭塞性(SMAO)休克前后血清蛋白质组学变化及其在SMAO休克发生中的作用。方法:应用家兔肠系膜上动脉夹闭法复制家兔SMAO休克模型,在此基础上通过双向电泳分离家兔SMAO休克前后血清中的蛋白,找出凝胶上的差异蛋白点,用基质辅助激光解吸/电离串联飞行时间质谱技术进行鉴定,并通过生物信息学对差异蛋白的功能进行分析。结果:在家兔SMAO休克前后血清双向电泳图谱中发现19个差异蛋白点,其中11个蛋白质点在SMAO休克后血清中表达明显上调;8个蛋白质点在SMAO休克后血清中表达明显下调。从中选取4个差异最明显的点经基质辅助激光解吸/电离串联飞行和数据库搜索共鉴定出符合条件的2个差异蛋白点,为对氧磷酶和触珠蛋白,均在SMAO休克后血清中含量增高。结论:家兔SMAO休克前后血清蛋白质组会发生明显变化,对氧磷酶和触珠蛋白可能参与了SMAO休克后机体的代偿调节。

Objective：To explor the changes of serum proteomics in rabbits superior mesenteric artery occlusion（SMAO） shock as well as its possible effect in SMAO shock.Methods：SMAO shock model in rabbits were induced by occlusion of the superior mesenteric artery,serum samples were obtained from rabbits before and after SMAO shock,proteins in samples were separated by two-dimensional electrophoresis（2-DE）,spots in the 2-DE map were detected and evaluated by PDQuest software 8.0.The spots with different expression level were subjected to matrix assisted laser desorption/ionization-time of flight-time of flight-mass spectrometry（MALDI-TOF-TOF-MS） for identification.,the protein database was searched to further characterized the differential proteins.Results：19 differential protein spots were screened out in the 2-DE maps,11 proteins were up-regulated and 8 proteins were down-regulated in SMAO shock rabbits＇s serum.4 of the 19 differential protein spots were selected for MALDI-TOF-TOF-MS study,and 2 of the 4 differential protein spots were identified satisfactoryly as paraoxonase and haptoglobin,which content were increased in rabbits＇s serum after SMAO shock.Conclusion：Serum proteomics of rabbit change remarkablely before and after SMAO shock,paraoxonase and haptoglobin may be associated with the compensation after SMAO shock.