联合补充维生素B_1、B_2、PP对急性低氧暴露小鼠物质代谢的改善作用

Improvement effect of vitamins B_1,B_2 and PP supplementation on substance metabolism of mice exposed to acute hypoxia

目的:观察联合补充维生素B1、B2、PP对急性低氧暴露小鼠碳水化合物、脂类、蛋白质以及能量代谢的改善作用。方法:将雄性昆明小鼠随机分为正常对照组、低氧对照组2、倍、4倍及8倍维生素B1、B2、PP补充组,各组动物以相应饲料喂养2周后,除正常对照组外,其他各组均以减压缺氧方式模拟6 000 m高度停留8 h,分析血糖、肝糖原、血清丙酮酸、血乳酸、血清尿素氮、血清游离脂肪酸和β-羟丁酸、全血三磷酸腺苷(ATP)含量的变化。结果:急性低氧暴露后,小鼠血糖、肝糖原、血清丙酮酸、血乳酸、血清游离脂肪酸,β-羟丁酸,尿素氮含量均显著上升(P<0.05),全血ATP含量下降;补充维生素B1、B2、PP对急性低氧导致的以上生化指标的变化有一定的改善作用。结论:急性低氧暴露后,机体三大营养素代谢发生改变,补充维生素B1、B2、PP可以在一定程度上缓解急性低氧对机体带来的代谢变化,4倍补充剂量效果较好。

Objective：To explore the improvement effect of vitamins B1,B2,PP supplementation to the metabolism changes of carbohydrates,lipids,protein and energy in mice exposed to acute hypoxia.Methods：Fifty male Kunming mice were randomly divided into normal,acute hypoxia,acute hypoxia plus 2 times,4 times and 8 times vitamins B1,B2,PP supplemented groups.All mice were fed corresponding diets for two weeks and then except the normal group were exposed to a simulated altitude of 6 000 meters for 8 hours.The changes of glucose,pyruvate,lactate,urea nitrogen,free fatty acids and β-hydroxybutyric acid from serum,liver glycogen and blood adenosine triphosphate（ATP） concentration were measured. Results：After being exposed to acute hypoxia,the mice glucose,liver glycogen,pyruvate,lactate,free fatty acids,β-hydroxybutyric acid and urea nitrogen level were increased significantly（P0.05）,while blood ATP concentration was decreased.In the vitamins B1,B2 and PP supplemented groups,these changes were improved.Conclusion：The significant changes in carbohydrate,lipid and protein metabolism were observed in mice exposed to acute hypoxia,and the supplementation of vitamins B1,B2 and PP was proved to be beneficial in improving some metabolic pathways.It is suggested that the supplemented dose of four times was good.