灌胃和皮下注射脂肪膜蛋白卵黄抗体对大鼠生长和脂肪沉积的影响

Intragastric and subcutaneous administration of yolk antibodies against adipocyte plasma membrane proteins on growth and fat deposition in rats

目的:研究脂肪膜卵黄抗体不同处理对大鼠生长和脂肪沉积的影响。方法:选用140 g左右雌性SD大鼠96只,随机分成4组,分别灌胃阴性卵黄和含脂肪细胞膜蛋白抗体的阳性卵黄;皮下注射阴性卵黄和含脂肪细胞膜蛋白抗体的阳性卵黄。灌胃每3 d给予1 ml卵黄,皮下注射连续4 d经背部皮下多点注射1 ml卵黄,1月后同方式加强1次。75 d后屠宰并采集血样测定。结果:阳性卵黄处理后大鼠体重和摄食量无显著差异。灌胃阳性卵黄降低肠系膜脂指数、子宫周脂指数和肾脂肪囊指数(P<0.05);降低血清甘油三酯(P<0.05),升高血清游离脂肪酸(P<0.01);降低血清Leptin、胰岛素和TNF-α水平(P<0.01或P<0.05),但对腓肠肌生长、血清总胆固醇无显著影响。皮下注射阳性卵黄提高腓肠肌指数(P<0.05);降低血清甘油三脂(P<0.01);降低血清Leptin(P<0.01),升高血清TNF-α(P<0.05);而对脂肪沉积、血清游离脂肪酸、总胆固醇和胰岛素无显著影响。结论:脂肪细胞膜蛋白卵黄抗体能有效改善机体组成,灌胃的效果优于皮下注射。

Objective：To study the effects of different administrations of antibodies against adipocyte plasma membrane proteins on growth and fat deposition in rats.Method：Ninety six female SD rats that weighed approximately 140 g were allotted randomly into four groups which were given negitive control yolk and positive yolk containing antibody（IgY） against adipocyte plasma membrane（APM） proteins by intragastric administration（i.g.） and subcutaneous injection（s.c.） respectively.Rats were given 1 ml of yolk for every three days in i.g.groups.Rats were given 1 ml of yolk for 4 consecutive days,and the procedure was repeated after one month.The trial lasted for 75 days after which rats were slaughtered for carcass analysis and sampling.Results：The body weight gain and food intake of rats were not different between treatment and control groups.In i.g.goups,positive yolk decreased mesemteric fat index,paramertrial fat index and perirenal fat index（P0.05）,reduced serum triglycerides（P0.05） and increased serum free fatty acids（FFA）（P0.01）,and also decreased serum leptin,insulin and TNF-α levels（P0.01 or P0.05）,but did not affect gastrocnemius muscle index and serum cholesterol.In s.c.groups,positive yolk increased gastrocnemius muscle index（P0.05）,reduced serum triglycerides and serum leptin（P0.01）,increased serum TNF-α（P0.05）,but did not affect adipose tissue depots,serum FFA,cholesterol and insulin.Conclusion：Administration of yolk antibody against APM proteins could effectively improve body composition of rats,and the treatment by intragastric administration could give better effect than by subcutaneous injection.