模拟65msw氦氧饱和潜水对人体氧化应激的影响

Effect of a simulated He-O_2 saturation dive to 65 msw on oxidative stress in humans

目的:探讨模拟65 msw饱和潜水暴露对机体氧化应激的影响。方法:7名健康男性潜水员参加65 msw饱和潜水,在暴露前、中、后取24 h尿检测超氧化物歧化酶(SOD)、丙二醛(MDA)、总氨基酸(T-AA)、总抗氧化能力(T-AOC)含量,同时监测24 h尿量及体重变化。结果:总氨基酸含量在减压末期比基础值显著增高,减压后1周恢复至正常水平;SOD、MDA、T-AOC在各时间点无明显变化。进舱后第1天尿量比进舱前基础值明显减少,随后逐渐恢复。加压后第2天开始体重逐渐增高,减压结束时比进舱前体重明显增高。结论:模拟65 msw饱和潜水暴露未造成参试潜水员明显氧化损伤。在饱和潜水试验中实时监测24 h尿量及氧化应激程度对防止机体损伤具有重要意义。

Objective：To investigate whether a simulated He-O2 saturation dive to 65 msw would affect oxidative balance in humans.Methods：Seven divers participated in a simulated saturation dive to 0.75MPa（65msw）.24-h urine samples were collected twice before,twice during,and twice after the dive,then were analyzed for contents of superoxide dismutase（SOD）,malondialdehyde（MDA）,total amino acid（T-AA） and total anti-oxidant capacity（T-AOC）.Meanwhile,total urine volume and body weight were measured.Results：The content of T-AA was higher（P0.05） than the base value in final decompression,but reverse to normal at one week after decompression.There were no changes in contents of SOD,MDA and TAOC during and after the dive compared with their basic value.Total urine volume was lower（P0.05,vs basic value） at first day in chamber,then returned to normal.Body weight gradually increased after compression till the end of decompression（higher than basic value,P0.05）.Conclusion：These data indicate that simulated saturation dive to 65 msw may not induce obvious oxidative damage,but it is necessary to monitor 24-h urine volume and oxidative sress by time in order to prevent from tissue injury.