摘要
采用Sevage法脱蛋白、乙醇沉淀、DEAE-纤维素离子交换柱层析和Sephadex G-200分子筛层析法,从Viili中分离纯化得到Viili中乳酸菌胞外多糖(EPS)。采用秀丽线虫进行寿命实验,观察Viili EPS延长秀丽线虫寿命的效果,在20℃下,饲喂Viili EPS 200 mg.L-1组秀丽线虫的平均寿命为17.13±0.53 d,与空白组相比,Viili EPS能够显著延长线虫的平均寿命(p<0.001)。
The extracelluar polysaccharide(EPS) from Viili was obtained by Sevage method,ethanol deposition,the DEAE gel chromatography column and Sephadex G-200 chromatography column.Then the effect of Viili EPS on longevity of Caenorhabditis elegans was detected by lifespan test.At 20℃,the mean lifespan of C.elegans was 17.13±0.53 d with Viili EPS 200 μg·mL-1.Compared with the control,the Viili EPS-treated groups showed significant increase in the mean lifespan of C.elegans(p 0.001).
出处
《氨基酸和生物资源》
CAS
2011年第2期64-67,共4页
Amino Acids & Biotic Resources
关键词
胞外多糖
纯化
寿命
秀丽线虫
Viili
extracelluar polysaccharide(EPS)
purification
lifespan
Caenorhabditis elegans
Viili