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Viili中胞外多糖的分离纯化及对秀丽线虫寿命的影响 被引量:4

Isolation,Purification of Extracellular Polysaccharide from Viili and Its Effect on the Lifespan of Caenorhabditis elegans
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摘要 采用Sevage法脱蛋白、乙醇沉淀、DEAE-纤维素离子交换柱层析和Sephadex G-200分子筛层析法,从Viili中分离纯化得到Viili中乳酸菌胞外多糖(EPS)。采用秀丽线虫进行寿命实验,观察Viili EPS延长秀丽线虫寿命的效果,在20℃下,饲喂Viili EPS 200 mg.L-1组秀丽线虫的平均寿命为17.13±0.53 d,与空白组相比,Viili EPS能够显著延长线虫的平均寿命(p<0.001)。 The extracelluar polysaccharide(EPS) from Viili was obtained by Sevage method,ethanol deposition,the DEAE gel chromatography column and Sephadex G-200 chromatography column.Then the effect of Viili EPS on longevity of Caenorhabditis elegans was detected by lifespan test.At 20℃,the mean lifespan of C.elegans was 17.13±0.53 d with Viili EPS 200 μg·mL-1.Compared with the control,the Viili EPS-treated groups showed significant increase in the mean lifespan of C.elegans(p 0.001).
出处 《氨基酸和生物资源》 CAS 2011年第2期64-67,共4页 Amino Acids & Biotic Resources
关键词 胞外多糖 纯化 寿命 秀丽线虫 Viili extracelluar polysaccharide(EPS) purification lifespan Caenorhabditis elegans Viili
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共引文献106

同被引文献102

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