刚竹秆褐腐病病原形态及分子鉴定

Morphological and molecular identification of bamboo culm brown rot

刚竹秆褐腐病在南京地区发生较普遍,影响竹林生长。其主要危害刚竹属的淡竹(Phyl-lostachys glauca)、黄槽刚竹(Ph.viridis f.houzeauana),其中以淡竹受害最为严重。笔者通过对刚竹秆褐腐病病组织分离培养、人工接种试验、分离菌形态学观察及采用通用引物ITS1/ITS4扩增,对扩增出的约559 bp的片段进行ITS序列分子鉴定,最终将在淡竹和黄槽刚竹等上发生的病原鉴定为木贼镰刀菌(Fusarium equiseti(Corda)Sacc.)。

Bamboo culm brown rot occurs generally,especially in Nanjing,and influences the growth of bamboo such as Phyllostachys glauca and Ph.viridis f.houzeauana,especially for the former.There was no any reports about Ph.glauca infected the disease previously.By isolation and culture of diseased tissue、artificial inoculation test,morphological observation of isolated strain and amplification with universal primer ITS1/ITS4,finally molecular identification with fragment amplified of 559 bp by ITS analysis,the pathogen of Ph.glauca and Ph.viridis f.houzeauana was identified as Fusarium equiseti（Corda） Sacc..The research result could provide the reference basis for the effective monitoring and control of the disease.