抗松材线虫病赤松组培苗的生根与移栽研究

Study on rooting and transplanting of the tissue-cultured plantlets from Pinus densiflora with good resistance to pine wilt disease

以抗病性较好但不易生根的抗松材线虫病赤松无性系为试验材料,对影响组培苗生根移栽的因素进行了研究。结果表明:将待生根的丛生芽置于17℃条件下处理5 d后,不定根诱导率最高达93%;在生根培养基中添加30～50 mg/L香蕉汁有利于不定根诱导;对移栽基质进行高温灭菌或0.2%高锰酸钾消毒2次的处理均可有效提高组培苗移栽成活率;喷施营养液对组培苗的长势具有促进作用。

Factors influencing rooting and transplanting of tissue-cultured plantlets from Pinus densiflora with excellent resistance to pine wilt disease but poor ability of rooting were investigated.The results showed that the induction rate of the adventitious root reached 93% after treating the shoots at 17 ℃ for 5 days,and it was beneficial for adventitious root induction as adding 30—50 mg/L banana juice into the rooting medium with a controlled temperature of 25 ℃.To sterilize the transplant matrix at high temperature or with 0.2% KMnO4 for two times would be helpful for improving the survival ratio of the tissue-cultured plantlets.Spraying nutrition solution could stimulate the plantlets growth.This research contributed to improve the root induction and transplanting survival rate,which provided factory style breeding system and practice evidences.