抑癌基因ARHI在子宫内膜异位症及非子宫内膜异位症患者中差异表达的意义

Differential expression of the anti-oncogene ARHI between patients with and without endometriosis

目的研究子宫内膜异位症(endometriosis,EMs)组织中抑癌基因ARHI的mRNA和蛋白表达情况。方法利用RT-PCR和Western Blot免疫印迹的方法,对EMs的异位内膜组织、在位内膜组织和非EMs在位内膜组织(正常内膜组织)进行mRNA和蛋白表达的半定量分析。利用原位杂交和免疫组化技术对研究各组进行定性及定位分析。结果 (1)ARHI mRNA和蛋白在各组中均有表达,异位内膜的表达高于在位内膜和正常内膜,差异显著(P<0.005),在位内膜组与正常对照组比较,ARHI的表达无明显差异(P>0.05);(2)原位杂交阳性率分别为非EMs组97.3%,EMs异位内膜组100%,EMs在位内膜组93.8%;(3)免疫组化阳性率分别为非EMs组93.2%,EMs异位内膜组100%,EMs在位内膜组92.3%;(4)ARHI的DNA与蛋白表达一致;(5)5例恶变的异位内膜ARHI免疫组化检测,4例呈阴性,1例呈弱阳性表达。结论抑癌基因ARHI在子宫内膜组织中均表达,且在异位内膜中呈表达上调,而在恶变的异位内膜中多呈阴性表达,提示ARHI基因可能与子宫内膜异位症不孕及恶变相关。

Objective To investigate the expression of the anti-oncogene ARHI in the endometriotic tissue and explore its clinical significance.Methods A semiquantitative analysis of the expression of ARHI mRNA and protein in the ectopic and eutopic endometrium of women with endometriosis was conducted using RT-PCR and Western blotting in comparison with that in the endometrium of women without endometriosis.Immunohistochemisty and in situ hybridization were performed for semiqualitative analysis and localization of ARHI expression.Results The expression levels of ARHI mRNA and protein differed significantly between the groups.ARHI was expressed at significantly higher levels in ectopic endometrium than in eutopic and normal endometrium（P0.005） ,but showed no significant difference between the latter two tissues（P0.05） .The positivity rate ARHI DNA was 97.3% in the endometrium of women without endometriosis,100% in the ectopic endometrium and 93.8% in the eutopic endometrium of women with endometriosis.Immunohistochemisty showed an ARHI positivity of 93.2% in the endometrium of women without endometriosis,100% in the ectopic endometrium and 92.3% in the eutopic endometrium of women with endometriosis.The expression patterns of ARHI DNA and protein were consistent.Immunohistochemisty in 5 cases of malignant endometriosis showed negative ARHI expression in 4 cases and weak positivity in 1 case.Conclusion ARHI expressions are present in the endometrium and up-regulated in ectopic endometrium,whereas in the ectopic endometrium of patients with malignant endometriosis its expression is often negative,suggesting a role of ARHI in infertility and tumorigenesis of endometriosis.