摘要
分生结节是植物组织离体发育的一种特殊状态,也是一种有价值的植株再生途径。它的外观与体细胞胚有相似之处,但其结构和发育过程上有明显区别。分生结节可以通过液体培养实现高效增殖,并能在长期增殖后保持遗传稳定性和分化能力,在植物微繁殖、次生代谢物生产、植物生长发育的机制等研究领域具有重要的理论和应用价值。该文结合国内外最新研究结果,综合论述了分生结节发生和植株再生的过程及其影响因素,并通过与体细胞胚等其它植株再生途径相比较,分析了分生结节的独特之处及其广阔的应用前景。
Meristematic nodules are tissues with distinct status during plant in vitro development and thus provide an important regeneration pathway via organogenesis.They are similar to somatic embryos in appearance but differ in structures and developmental processes.Meristematic nodules go through unique processes in their development;therefore,they can be multiplied by repeat liquid culture.The genetic stability and differentiation competence of meristematic nodules are well kept.Meristematic nodules are valuable both theoretically and practically in plant micropropagation,secondary metabolite production,and research into plant development mechanisms.This paper reviews the processes and impact factors of meristematic nodule initiation and plant regeneration and compares the characteristics and application prospects of this pathway with somatic embryogenesis.
出处
《植物学报》
CAS
CSCD
北大核心
2011年第3期350-360,共11页
Chinese Bulletin of Botany
基金
北京市教委共建项目(2009)
林业科学重点科研项目(No.2006-40)
关键词
分生结节
微繁殖
器官发生
植株再生
体细胞胚
meristematic nodule
micropropagation
organogenesis
plant regeneration
somatic embryogenesis
