HepG2细胞系载脂蛋白B表达的调控研究

Study on the Effects of IL-11 and Ethanol on the Expression and Secrction of apoB in HepG2 Cells

探讨白细胞介素－11（IL－11）及乙醇对人肝肿瘤细胞系（HepG2）载脂蛋白B（apoB）mRNA表达及蛋白分泌的作用。培养HepG2细胞，分组加入不同浓度IL－11进行浓度试验，收集培养液及细胞。用火箭电泳法及Northernblot分别检测培养液中apoB含量及HepG2细胞apoBmRNA的表达。IL－11及乙醇抑制试验按同法分组进行。结果IL－11能抑制HepG2细胞apoB表达与分泌，且呈量效正相关。IL－11能明显抑制乙醇的正向作用。说明IL－11能调节HepG2细胞apoBmRNA表达与分泌，可参与调节机体脂质代谢。

To study the effects of interleukin (IL)-11 and ethanol on the expression and secretion of apoB in HepG2 cells. HepG2 cells were cultured in DMEM and divided into 4 groups with addition of different concentrations of IL-11. Medium was collected for measuring apoB by using electroimmunoassays, and cells harvested to detect the expression of apoB mRNA by using Northern blot analysis. Ethanol inhibiting experiment by IL-11 was performed with the same methods. It was found that IL-11 could inhibit the expression of apoB mRNA and its secretion in HepG2 cells in a dose-dependent manner and obviously inhibit the positive action of ethanol, suggesting that IL-11 could regulate the expression and secretion of apoB in HepG2 and may play some role in the apolipoprotein metabolism of liver cells.